Creatine Kinase, Brain (CKB) antibody

Antigen: Creatine Kinase, Brain (CKB)

Synonyms: B-CK, CKBB, Bck, Ck3, Ck-3, Ckbb, bck, ckb, cb594, wu:fj36f10, wu:fj37d11, MGC53426, CKB, b-ck, ckbb, Ckbr, RATCKBR

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN185601

Additional Information

Alternative name: CKB / Brain Creatine Kinase

Gene ID: 1152, 12709 (mouse)

Immunogen: C-KPSDEHKTDLNPDN

Format: Affinity-purified

Description: All names and symbols: CKB, brain creatine kinase, creatine kinase, brain , B-CK, CKBB , creatine kinase B-chain, creatine kinase-B. Official Symbol: CKB. RefSeq number(s): NP_001814.2

Specificity: Human, Mouse, Rat, Dog

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:2,000. Western Blot: Approx 45-48kDa band observed in human brain lysates (calculated MW of 42.6kDa according to NP_001814.2). Recommended concentration: 0.1-0.3µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN185601 (0.1µg/ml) staining of human brain lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Castegna, Aksenov, Aksenova et al.: "Proteomic identification of oxidatively modified proteins in Alzheimer's disease brain. Part I: creatine kinase BB, glutamine synthase, and ubiquitin carboxy-terminal hydrolase L-1." in: Free radical biology & medicine, Vol. 33, Issue 4, pp. 562-71, 2002 (PubMed).

Balasubramani, Day, Schoen et al.: "Altered expression and localization of creatine kinase B, heterogeneous nuclear ribonucleoprotein F, and high mobility group box 1 protein in the nuclear matrix associated with colon cancer." in: Cancer research, Vol. 66, Issue 2, pp. 763-9, 2006 (PubMed).