Creatine Kinase, Brain (CKB) (AA 7-381) antibody

Catalog No. ABIN5692824

Product Details

Target:

Creatine Kinase, Brain (CKB)

Binding Specificity: AA 7-381

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Brand: Picoband™

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for CKB detection. Tested with WB, IHC-P, Direct ELISA in Human,Mouse,Rat.

Immunogen: E. coli-derived human CKB recombinant protein (Position: H7-K381).

Application Details

Application Notes:

Recommended Detection Systems: Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ABIN921124) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).

Application Details: Western blot, 0.1-0.5&mu,g/mL
Immunohistochemistry(Paraffin-embedded Section), 0.5-1&mu,g/mL
Direct ELISA, 0.1-0.5&mu,g/mL

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Target Details

Target:

Creatine Kinase, Brain (CKB)

Alternative Name: CKB (CKB Antibody Abstract)

Synonyms: B-CK, CKBB, b-ck, ckbb, CKB, Bck, Ck-3, Ck3, Ckbb, bck, ckb, cb594, wu:fj36f10, wu:fj37d11, Ckbr, creatine kinase B, creatine kinase, brain L homeolog, creatine kinase B-type, creatine kinase, brain, creatine kinase, brain b, CKB, ckb.L, Ckb, ckb, ckbb

Background:

Synonyms: Creatine kinase B-type, B-CK, Creatine kinase B chain, CKB, CKBB

Background: Brain-type creatine kinase also known as CK-BB is a creatine kinase that in humans is encoded by the CKB gene. The protein encoded by this gene is a cytoplasmic enzyme involved in energy homeostasis. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in brain as well as in other tissues, and as a heterodimer with a similar muscle isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family. A pseudogene of this gene has been characterized.

UniProt: P12277