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Antibodies
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anti-Lymphoid Enhancer-Binding Factor 1 (LEF1) antibody
Lymphoid Enhancer-Binding Factor 1 (LEF1) antibody
| Antigen |
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| Synonyms |
FLJ46390, TCF1ALPHA, DKFZp586H0919, LEF-1, LEF1 |
| Clonality |
Polyclonal |
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Host
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Reactivity
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Application
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Western Blotting (WB) (51), Immunofluorescence (IF) (27), Flow Cytometry (FACS) (19), Enzyme Immunoassay (EIA) (11), ELISA (9), Immunohistochemistry (IHC) (4), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (4), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoprecipitation (IP) (2), ELISA (Detection) (1), Gel Shift (GS) (1), Immunocytochemistry (ICC) (1), Immunoelectron Microscopy (IEM) (1)
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| Catalog no. |
ABIN185607 |
| Quantity |
100 ug (Variants) |
| Price |
501.80 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 5 Business Days |
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Alternative name
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LEF1
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Gene ID
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51176, 16842 (mouse), 161452 (rat)
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Immunogen
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C-QHEQRKEQEPKRPH
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Format
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Affinity-purified
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Description
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All names and symbols: LEF1, lymphoid enhancer-binding factor 1 , HGNC:6551, DKFZp586H0919, TCF1ALPHA , lymphoid enhancer binding factor-1. Official Symbol: LEF1. RefSeq number(s): NP_057353.1
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Specificity
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Human, Mouse, Rat, Dog 399 aa
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Application Notes
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Peptide ELISA: antibody detection limit dilution 1:32,000. Approx 48kDa band observed in lysates of cell lines Daudi and Jurkat (calculated MW of 44.2kDa according to NP_057353.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Yasumoto et al , EMBO J. 2002 Jun 3,21(11):2703-14., PMID: 12032083). Recommended concentration: 1-3µg/ml.
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Purification
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Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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Buffer
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0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
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Storage
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Aliquot and store at -20°C. Minimize freezing and thawing.
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Research Area
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Transcription Factors, Wnt, Lineage Markers
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Restrictions
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For Research Use only
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Publications
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Waterman: "Lymphoid enhancer factor/T cell factor expression in colorectal cancer." in: Cancer metastasis reviews, Vol. 23, Issue 1-2, pp. 41-52, 2004 (PubMed).
Radtke, Clevers: "Self-renewal and cancer of the gut: two sides of a coin." in: Science (New York, N.Y.), Vol. 307, Issue 5717, pp. 1904-9, 2005 (PubMed).
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Alternatives for antigen "Lymphoid Enhancer-Binding Factor 1 (LEF1)", type "Antibodies"
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Hosts
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Mouse (34), Rabbit (25), Goat (6), Sheep (5)
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Reactivities
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Human (62), Mouse (Murine) (30), Rat (Rattus) (24), Dog (Canine) (21), Cow (Bovine) (17), Pig (Porcine) (16), Chicken (15), Rabbit (15), Horse (Equine) (1)
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Applications
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Western Blotting (WB) (51), Immunofluorescence (IF) (27), Flow Cytometry (FACS) (19), Enzyme Immunoassay (EIA) (11), ELISA (9), Immunohistochemistry (IHC) (4), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (4), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoprecipitation (IP) (2), ELISA (Detection) (1), Gel Shift (GS) (1), Immunocytochemistry (ICC) (1), Immunoelectron Microscopy (IEM) (1)
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Conjugates
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Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
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Epitopes
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HMG Binding Domain (2), all isoforms (1), alternate exon (1), transactivation domain (1)
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Alternatives 
