LEF1 antibody (AA 1-399)

Catalog No. ABIN565556

There are 2+ publications for this product available. The Mouse Monoclonal anti-LEF1 antibody is suitable to detect LEF1 in samples from Human. It has been validated for WB, ELISA and RNAi.

Quick Overview for LEF1 antibody (AA 1-399) (ABIN565556)

Target: LEF1 (Lymphoid Enhancer-Binding Factor 1 (LEF1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This LEF1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, RNA Interference (RNAi)

Clone: 3H5

Product Details anti-LEF1 Antibody

Binding Specificity: AA 1-399

Purpose: Mouse monoclonal antibody raised against a full length recombinant LEF1.

Sequence: MPQLSGGGGG GGGDPELCAT DEMIPFKDEG DPQKEKIFAE ISHPEEEGDL ADIKSSLVNE SEIIPASNGH EVARQAQTSQ EPYHDKAREH PDDGKHPDGG LYNKGPSYSS YSGYIMMPNM NNDPYMSNGS LSPPIPRTSN KVPVVQPSHA VHPLTPLITY SDEHFSPGSH PSHIPSDVNS KQGMSRHPPA PDIPTFYPLS PGGVGQITPP LGWQGQPVYP ITGGFRQPYP SSLSVDTSMS RFSHHMIPGP PGPHTTGIPH PAIVTPQVKQ EHPHTDSDLM HVKPQHEQRK EQEPKRPHIK KPLNAFMLYM KEMRANVVAE CTLKESAAIN QILGRRWHAL SREEQAKYYE LARKERQLHM QLYPGWSARD NYGKKKKRKR EKLQESASGT GPRMTAAYI

Cross-Reactivity: Human

Characteristics: Antibody Reactive Against Recombinant Protein.

Immunogen: LEF1 (AAH50632, 1 a.a. ~ 399 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: In 1x PBS, pH 7.4

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References for anti-LEF1 antibody (ABIN565556)

Jamieson, Sharma, Henderson: "Regulation of ?-catenin nuclear dynamics by GSK-3? involves a LEF-1 positive feedback loop." in: Traffic (Copenhagen, Denmark), Vol. 12, Issue 8, pp. 983-99, (2011) (PubMed).

Hebenstreit, Giaisi, Treiber, Zhang, Mi, Horejs-Hoeck, Andersen, Krammer, Duschl, Li-Weber: "LEF-1 negatively controls interleukin-4 expression through a proximal promoter regulatory element." in: The Journal of biological chemistry, Vol. 283, Issue 33, pp. 22490-7, (2008) (PubMed).

Target Details for LEF1

Target: LEF1 (Lymphoid Enhancer-Binding Factor 1 (LEF1))

Alternative Name: LEF1

Background: Full Gene Name: lymphoid enhancer-binding factor 1
Synonyms: DKFZp586H0919,TCF1ALPHA

Gene ID: 51176

Pathways: WNT Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Hormone Metabolic Process, Nuclear Hormone Receptor Binding, Chromatin Binding