Vesicle Transport through Interaction with T-SNAREs Homolog 1B (Yeast) (VTI1B) antibody

Antigen: Vesicle Transport through Interaction with T-SNAREs Homolog 1B (Yeast) (VTI1B)

Synonyms: VTI1, VTI2, VTI1L, VTI1-LIKE, GES30, SNARE, MVti1b, AU015348, Vti1-rp1, MGC63525, zgc:63525, VTI1B, MGC137210

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN185615

Additional Information

Alternative name: VTI1B

Gene ID: 10490, 53612 (mouse)

Immunogen: C-RDFDEKQQEANET

Format: Affinity-purified

Description: All names and symbols: VTI1B, vesicle transport through interaction with t-SNAREs homolog 1B (yeast) , HGNC:17793, VTI1, VTI1L, VTI2 , v-SNARE, vesicle transport through interaction with t-SNAREs 1B, vesicle-associated soluble NSF attachment protein receptor. Official Symbol: VTI1B. RefSeq number(s): NP_006361.1

Specificity: Human, Mouse, Rat, Dog

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx 26-28kDa band observed in Human Duodenum, Kidney and Testes lysates (calculated MW of 26.7kDa according to NP_006361.1). Recommended concentration: 1-3µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Signaling

Restrictions: For Research Use only

Images

ABIN185615 (1 ug/ml) staining of Human Duodenum lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Murray, Wylie, Khromykh et al.: "Syntaxin 6 and Vti1b form a novel SNARE complex, which is up-regulated in activated macrophages to facilitate exocytosis of tumor necrosis Factor-alpha." in: The Journal of biological chemistry, Vol. 280, Issue 11, pp. 10478-83, 2005 (PubMed).