CYP17A1 antibody (Internal Region)

Catalog No. ABIN185617

This Goat Polyclonal antibody specifically detects CYP17A1 in WB, ELISA and IHC. It exhibits reactivity toward Human.

Quick Overview for CYP17A1 antibody (Internal Region) (ABIN185617)

Target: CYP17A1 (Cytochrome P450, Family 17, Subfamily A, Polypeptide 1 (CYP17A1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This CYP17A1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-CYP17A1 Antibody

Binding Specificity: Internal Region

Purpose: CYP17A1

Sequence: LFKDGDQKLE K

Cross-Reactivity: Cat, Dog, Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-LFKDGDQKLEK, from the internal region of the protein sequence according to NP_000093.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Adenal Gland shows strong granular staining of the cytoplasm in medulla cells. Recommended concentration: 3-5 μg/mL.

Western Blot: Approx 60 kDa band observed in Human Ovary lysates (calculated MW of 57.4 kDa according to NP_000093.1). Recommended concentration: 0.1-0.3 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:64000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for CYP17A1

Target: CYP17A1 (Cytochrome P450, Family 17, Subfamily A, Polypeptide 1 (CYP17A1))

Alternative Name: CYP17A1

Background: CYP17A1, cytochrome P450, family 17, subfamily A, polypeptide 1 , HGNC:2593, CPT7, CYP17, P450C17, S17AH, cytochrome P450, family 17, cytochrome P450, subfamily XVII (steroid 17-alpha-hydroxylase), adrenal hyperplasia, cytochrome p450 XVIIA1, steroid 17-a

Gene ID: 1586

NCBI Accession: NP_000093

Pathways: Metabolism of Steroid Hormones and Vitamin D, Steroid Hormone Biosynthesis, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, C21-Steroid Hormone Metabolic Process, Cellular Response to Molecule of Bacterial Origin