Casein Kinase 1 delta antibody (Internal Region)

Catalog No. ABIN185622

This anti-Casein Kinase 1 delta antibody is a Goat Polyclonal antibody detecting Casein Kinase 1 delta in WB, ELISA and IF. Suitable for Human and Mouse.

Quick Overview for Casein Kinase 1 delta antibody (Internal Region) (ABIN185622)

Target: Casein Kinase 1 delta (CSNK1D) (Casein Kinase 1, delta (CSNK1D))

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This Casein Kinase 1 delta antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Grade: Verified

Product Details anti-Casein Kinase 1 delta Antibody

Binding Specificity: Internal Region

Purpose: Casein Kinase 1, delta

Sequence: DREERLRHSR NPATR

Specificity: This antibody is expected to recognise both reported isoforms (NP_001884.2 and NP_620693.1)

Cross-Reactivity: Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-DREERLRHSRNPATR, from the internal region of the protein sequence according to NP_001884.2, NP_620693.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 48 kDa band observed in Mouse Brain lysates and in preliminary testing of U251 cell lysates (calculated MW of 47.3 kDa according to Mouse NP_620690.1). Recommended concentration: 0.1-0.3 μg/mL. Primary incubation 1 hour at room temperat

Peptide ELISA: antibody detection limit dilution 1:32000.

Comment:

Immunofluorescence: Expression of the protein seen in the Nucleus of U251 cells. Recommended concentration: 10μg/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for Casein Kinase 1 delta

Target: Casein Kinase 1 delta (CSNK1D) (Casein Kinase 1, delta (CSNK1D))

Alternative Name: CSNK1D

Background: Casein kinase 1, delta, HGNC:2452, CSNK1D, HCKID, CKIdelta

Gene ID: 1453, 104318, 64462

NCBI Accession: NP_001884, NP_620693

Pathways: Hedgehog Signaling, M Phase