Non-POU Domain Containing, Octamer-Binding (NONO) antibody

Antigen: Non-POU Domain Containing, Octamer-Binding (NONO)

Synonyms: HNT, FINB, LZ321, Zep-1, RREB-1

Clonality: Polyclonal

Host: Goat

Reactivity: Mouse (Murine)

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN185629

Additional Information

Alternative name: NONO / p54NRB

Gene ID: 4841, 53610 (mouse), 317259 (rat)

Immunogen: C-NRAAPGAEFAPNK

Format: Affinity-purified

Description: All names and symbols: NONO, P54NRB , non-POU domain containing, octamer-binding , HGNC:7871, NMT55, NRB54, P54, Nuclear RNA-binding protein, 54-kD, non-POU-domain-containing, octamer-binding, non-Pou domain-containing octamer (ATGCAAAT) binding protein. Official Symbol: NONO. RefSeq number(s): NP_031389.3

Specificity: Human, Mouse, Rat, Dog 471 aa

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: Approx 60kDa band observed in mouse brain lysates (calculated MW of 54.2kDa according to NP_031389.3). Recommended concentration: 0.1-0.3µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN185629 (0.1µg/ml) staining of mouse brain lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Brown, Ripperger, Kadener et al.: "PERIOD1-associated proteins modulate the negative limb of the mammalian circadian oscillator." in: Science (New York, N.Y.), Vol. 308, Issue 5722, pp. 693-6, 2005 (PubMed).