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Chat (mouse) antibody
| Antigen | Chat (mouse) |
| Clonality | Polyclonal |
| Host |
Goat |
| Reactivity |
Mouse (Murine), Rat (Rattus) |
| Application |
ELISA, Western Blotting (WB)
|
 |
1 reference available |
| Catalog no. | ABIN185635 |
| Quantity | 100 ug |
| Price | 501.80 $ Plus shipping costs $35.00 |
| Shipping to |
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| Availability | Ships within 5 Business Days |
Additional Information
| Gene ID | 12647 (mouse), 432359 (rat) |
| Immunogen | C-KERARGPTKPSNLD |
| Format | Affinity-purified |
| Description | All names and symbols: Chat, choline acetyltransferase , B230380D24Rik. Official Symbol: Chat. RefSeq number(s): NP_034021.1 |
| Specificity | This antibody is NOT expected to cross-react to Human CHAT. Mouse, Rat 645 aa |
Application Details
| Application Notes | Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx 75-80kDa band observed in Mouse and Rat Brain lysates (calculated MW of 72.3kDa according to NP_034021.1). Recommended concentration: 0.2-0.5µg/ml. |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. |
| Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
| Restrictions | For Research Use only |
Images
| ABIN185635 (0.2µg/ml) staining of Mouse Brain lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. |
Publications
| Publications |
Contestabile, Fila, Bartesaghi et al.: "Choline acetyltransferase activity at different ages in brain of Ts65Dn mice, an animal model for Down's syndrome and related neurodegenerative diseases." in: Journal of neurochemistry, Vol. 97, Issue 2, pp. 515-26, 2006 (PubMed).
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