Leptin Receptor (LEPR) antibody

Antigen: Leptin Receptor (LEPR)

Synonyms: OBR, CD295, obr, cd295, ob-rb

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN185639

Additional Information

Alternative name: Leptin Receptor

Gene ID: 3953

Immunogen: C-TQDDIEKHQSDAG

Format: Affinity-purified

Description: All names and symbols: LEPR , leptin receptor , CD295, OBR , OB receptor, LeprB. Official Symbol: LEPR. RefSeq number(s): NP_002294.2, NP_001003679.1, NP_001003680.1

Specificity: This antibody is expected to recognise all three reported isoforms (NP_002294.2, NP_001003679.1, NP_001003680.1). Human, Dog 1165 aa, 896 aa, 958 aa

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: Approx 150kDa band observed in Human Brain lysates (calculated MW of 132kDa according to NP_002294.2). Recommended concentration: 1-3µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Cardiovascular, Fatty Acids, Hormones, Receptors

Restrictions: For Research Use only

Images

ABIN185639 (1µg/ml) staining of Human Brain lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Snoussi, Strosberg, Bouaouina et al.: "Leptin and leptin receptor polymorphisms are associated with increased risk and poor prognosis of breast carcinoma." in: BMC cancer, Vol. 6, pp. 38, 2006 (PubMed).