Reactivity: Human, Rat
ELISA, FACS, IF (cc), IF (p), IHC (fro), IHC (p)
Titration of the Leptin Receptor antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,Flow Cytometry: 1:10-1:50,IHC (Paraffin): 1:50-1:100
For Research Use only
In 1X PBS, pH 7.4, with 0.09 % sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Aliquot the Leptin Receptor antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Leptin, an adipocyte-specific hormone, regulates adipose-tissue mass through hypothalamic effects on satiety and energy expenditure by acting through the leptin receptor (LEPR). LEPR is a single-transmembrane-domain receptor of the cytokine receptor family that is identical to the mouse diabetes (db) gene product. During weight loss, leptin levels decrease, whereas soluble LEPR levels and the receptor bound fraction of leptin increases. The presence of LEPR in the absorptive cells of the small intestine suggests that leptin may have a physiological role in the regulation of nutrient absorption.