Mitogen-Activated Protein Kinase Kinase Kinase 7 (MAP3K7) antibody

Antigen: Mitogen-Activated Protein Kinase Kinase Kinase 7 (MAP3K7)

Synonyms: TAK1, MEKK7, TGF1a, MGC110612, zgc:110612, si:ch211-225h9.1

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN185640

Additional Information

Alternative name: TAK1 / MAP3K7

Gene ID: 6885, 26409 (mouse)

Immunogen: C-AELDQDEKDQQNT

Format: Affinity-purified

Description: All names and symbols: MAP3K7 , mitogen-activated protein kinase kinase kinase 7 , TAK1, TGF1a , TGF-beta activated kinase 1, transforming growth factor-beta-activated kinase 1 . Official Symbol: MAP3K7 . RefSeq number(s): NP_003179.1, NP_663304.1

Specificity: This antibody is expected to recognise isoform A (NP_003179.1) and isoform B (NP_663304.1). Human, Mouse, Rat 579 aa, 606 aa, 518 aa, 491 aa

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:1,000. Western Blot: Approx 75kDa band observed in lysates of Human Colon and of cell lines NIH/3T3, A431 and HeLa (calculated MW of 64.2kDa according to NP_003179.1 and 67.2 according to NP_663304.1). R

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN185640 (1 ug/ml) staining of HeLa cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Shinohara, Yasuda, Aiba et al.: "PKC beta regulates BCR-mediated IKK activation by facilitating the interaction between TAK1 and CARMA1." in: The Journal of experimental medicine, Vol. 202, Issue 10, pp. 1423-31, 2005 (PubMed).

Omori, Matsumoto, Sanjo et al.: "TAK1 is a master regulator of epidermal homeostasis involving skin inflammation and apoptosis." in: The Journal of biological chemistry, Vol. 281, Issue 28, pp. 19610-7, 2006 (PubMed).