Silver Homolog (Mouse) (SILV) antibody

Antigen: Silver Homolog (Mouse) (SILV)

Synonyms: SI, SIL, ME20, PMEL, gp100, PMEL17, D12S53E, Silv, gp87, Pmel17, D12S53Eh, D10H12S53E, SILV, RPE1, Si, MMP115, silverb

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN185660

Additional Information

Alternative name: Silver homologue / Pmel 17

Gene ID: 6490, 20431 (mouse), 362818 (rat)

Immunogen: CPIGENSPLLSGQQ

Format: Affinity-purified

Description: All names and symbols: silver homolog (mouse) , PMEL17, SILV , D12S53E, ME20, SI, SIL, gp100 , Melanocyte protein mel 17, Pmel 17, Silver, mouse, homolog of, silver (mouse homolog) like, silver (mouse homolog)-like, silver homolog. Official Symbol: SILV . RefSeq number(s): NP_008859.1

Specificity: Human, Mouse, Rat

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: Approx 70-75kDa band observed in Human Skin lysates (calculated MW of 70.3kDa according to NP_008859.1). Recommended concentration: 1-3µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN185660 (1µg/ml) staining of Human Skin lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Fowler, Koulov, Alory-Jost et al.: "Functional amyloid formation within mammalian tissue." in: PLoS biology, Vol. 4, Issue 1, pp. e6, 2006 (PubMed).