Serotonin Receptor 3A (HTR3A) antibody

Antigen: Serotonin Receptor 3A (HTR3A)

Synonyms: HTR3, 5HT3R, 5-HT-3, 5-HT3A, 5-HT3R, 5-HT3, 5HT3, Htr3, MGC52789, HTR3A, Htr3a

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN185673

Additional Information

Alternative name: Serotonin receptor 3A / HTR3A

Gene ID: 3359

Immunogen: C-GPQDFEKSPRDR

Format: Affinity-purified

Description: All names and symbols: HTR3A, serotonin receptor, 5-hydroxytryptamine (serotonin) receptor 3A, 5-HT-3, 5-HT3A, 5-HT3R, 5HT3R, HTR3, 5-hydroxytryptamine (serotonin) receptor-3, 5HT3 serotonin receptor, Serotonin-gated ion channel receptor, truncated receptor, containing only 3 transmembrane domains. Official Symbol: HTR3A. RefSeq number(s): NP_998786.1, NP_000860.1

Specificity: This antibody is expected to recognise both reported isoforms (NP_998786.1 and NP_000860.1). Human 510 aa, 478 aa

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:128,000. Western Blot: Approx 55kDa band observed in Human Colon lysates (calculated MW of 55.3kDa according to NP_000860.1. Recommended concentration: 0.3-1µg/ml. A minor band of unknown identity was also consistently observed at 200kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN185673 (0.3µg/ml) staining of Human Colon lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Dremencov, Weizmann, Kinor et al.: "Modulation of dopamine transmission by 5HT2C and 5HT3 receptors: a role in the antidepressant response." in: Current drug targets, Vol. 7, Issue 2, pp. 165-75, 2006 (PubMed).