VHL antibody (Internal Region)

Catalog No. ABIN185675

The Goat Polyclonal anti-VHL antibody is suitable to detect VHL in samples from Human, Rat and Mouse. It has been validated for WB, IHC and ELISA.

Quick Overview for VHL antibody (Internal Region) (ABIN185675)

Target: VHL (Von Hippel-Lindau Tumor Suppressor, E3 Ubiquitin Protein Ligase (VHL))

Reactivity: Human, Rat, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This VHL antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-VHL Antibody

Binding Specificity: Internal Region

Purpose: VHL

Sequence: RSLVKPENYR RLD

Specificity: This antibody is expected to recognise both reported isoforms (NP_000542.1 and NP_937799.1).

Cross-Reactivity: Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-RSLVKPENYRRLD, from the internal region of the protein sequence according to NP_000542.1, NP_937799.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Kidney. Recommended concentration: 5 μg/mL.

Western Blot: Approx 17 kDa band observed in Human Ovary and Testis lysates and also in lysates of Mouse and Rat Testis and Brain and of all human and mouse cell lines tested (calculated MW of 19.7 kDa according to NP_937799.1). Recommended concentration:

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for VHL

Target: VHL (Von Hippel-Lindau Tumor Suppressor, E3 Ubiquitin Protein Ligase (VHL))

Alternative Name: VHL

Background: VHL, von Hippel-Lindau tumor suppressor, HRCA1, RCA1, VHL1 , elongin binding protein, von Hippel-Lindau syndrome

Gene ID: 7428, 22346, 24874

NCBI Accession: NP_000542, NP_937799

Pathways: Tube Formation, Signaling Events mediated by VEGFR1 and VEGFR2, Ubiquitin Proteasome Pathway