VHL antibody (AA 1-172)

Catalog No. ABIN3020968

Cited in 3+ publications. The Rabbit Polyclonal anti-VHL antibody (ABIN3020968) specifically detects VHL in WB, IHC and IF. The antibody is reactive with Human samples.

Quick Overview for VHL antibody (AA 1-172) (ABIN3020968)

Target: VHL (Von Hippel-Lindau Tumor Suppressor, E3 Ubiquitin Protein Ligase (VHL))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This VHL antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-VHL Antibody

Binding Specificity: AA 1-172

Sequence: MPRRAENWDE AEVGAEEAGV EEYGPEEDGG EESGAEESGP EESGPEELGA EEEMEAGRPR PVLRSVNSRE PSQVIFCNRS PRVVLPVWLN FDGEPQPYPT LPPGTGRRIH SYRVYTLKER CLQVVRSLVK PENYRRLDIV RSLYEDLEDH PNVQKDLERL TQERIAHQRM GD

Cross-Reactivity: Human, Mouse, Rat

Characteristics: Polyclonal Antibodies

Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-172 of human VHL (NP_937799.1).

Isotype: IgG

Application Details

Application Notes: WB,1:500 - 1:2000,IHC,1:50 - 1:200,IF,1:50 - 1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide,50 % glycerol, pH 7.3.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze / thaw cycles

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

References for anti-VHL antibody (ABIN3020968)

Liu, Cai, Hu, Mei, Zhang, Ouyang, Wang, Zhang, Xiao: "Forkhead Transcription Factor 3a (FOXO3a) Modulates Hypoxia Signaling via Up-regulation of the von Hippel-Lindau Gene (VHL)." in: The Journal of biological chemistry, Vol. 291, Issue 49, pp. 25692-25705, (2017) (PubMed).

Du, Zhang, Zhang, Ouyang, Wang, Liu, Li, Ji, Liu, Xiao: "pVHL Negatively Regulates Antiviral Signaling by Targeting MAVS for Proteasomal Degradation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 195, Issue 4, pp. 1782-90, (2015) (PubMed).

Wang, Zhang, Ji, Liu, Ouyang, Xiao: "The von hippel-lindau protein suppresses androgen receptor activity." in: Molecular endocrinology (Baltimore, Md.), Vol. 28, Issue 2, pp. 239-48, (2015) (PubMed).

Target Details for VHL

Target: VHL (Von Hippel-Lindau Tumor Suppressor, E3 Ubiquitin Protein Ligase (VHL))

Alternative Name: VHL

Background: Von Hippel-Lindau syndrome (VHL) is a dominantly inherited familial cancer syndrome predisposing to a variety of malignant and benign tumors. A germline mutation of this gene is the basis of familial inheritance of VHL syndrome. The protein encoded by this gene is a component of the protein complex that includes elongin B, elongin C, and cullin-2, and possesses ubiquitin ligase E3 activity. This protein is involved in the ubiquitination and degradation of hypoxia-inducible-factor (HIF), which is a transcription factor that plays a central role in the regulation of gene expression by oxygen. RNA polymerase II subunit POLR2G/RPB7 is also reported to be a target of this protein. Alternatively spliced transcript variants encoding distinct isoforms have been observed.,VHL,HRCA1,RCA1,VHL1,pVHL,PVHL,Epigenetics & Nuclear Signaling,Cancer,Tumor suppressors,Cell Biology & Developmental Biology,Apoptosis,Cell Cycle,Cell cycle inhibitors,Cell differentiation,Ubiquitin,Ubiquitin-Proteasome Signaling Pathway,Endocrine & Metabolism,Immunology & Inflammation,Cardiovascular,Angiogenesis,VHL

Molecular Weight: 18 kDa/19 kDa/24 kDa

Gene ID: 7428

UniProt: P40337

Pathways: Tube Formation, Signaling Events mediated by VEGFR1 and VEGFR2, Ubiquitin Proteasome Pathway