Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 1 (GRIN1) antibody

Antigen: Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 1 (GRIN1)

Synonyms: NR1, NMDA1, NMDAR1, grin1, nmda1, nmdar1, MGC145925, Nmdar1, GB19253, GRIN1, NMD-R1

Clonality: Polyclonal

Host: Goat

Reactivity: Rat (Rattus)

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN185678

Additional Information

Alternative name: NMDA receptor 1 / GRIN1

Gene ID: 2902, 14810 (mouse), 24408 (rat)

Immunogen: C-TQERVNNSNKKE

Format: Affinity-purified

Description: All names and symbols: GRIN1, NMDA receptor 1, glutamate receptor, ionotropic, N-methyl D-aspartate 1 , NMDA1, NMDAR1, NR1 , N-methyl-D-aspartate receptor channel, subunit zeta-1, glutamate [NMDA] receptor subunit zeta 1. Official Symbol: GRIN1. RefSeq number(s): NP_000823.4, NP_067544.1, NP_015566.1

Specificity: This antibody is expected to recognise all three reported isoforms (NP_000823.4, NP_067544.1, NP_015566.1). Human, Mouse, Rat, Dog

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:1,000. Western Blot: Approx 110kDa band observed in Rat Brain lysates (calculated MW of 105kDa according to NP_015566.1). Recommended concentration: 2-4µg/ml. A minor band of unknown identity was also consistently observed at 23-24kDa. This band was strong in Mouse Brain lysates and faint in Human Brain lysates. The band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN185678 (2µg/ml) staining of Rat Brain lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Rajji, Chapman, Eichenbaum et al.: "The role of CA3 hippocampal NMDA receptors in paired associate learning." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 26, Issue 3, pp. 908-15, 2006 (PubMed).