Catechol-O-Methyltransferase (COMT) (Internal Region) antibody

Antigen: Catechol-O-Methyltransferase (COMT)

Synonyms: Comt, D16Wsu103e, D330014B15Rik, COMT, mb-comt, s-comt, MGC140449, comt, MGC154444

Epitope: Internal Region

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB), Enzyme Immunoassay (EIA)

Catalog no.: ABIN185682

Additional Information

Alternative name: COMT

Gene ID: 1312

Swiss-Prot: NP_000745.1

Immunogen: Peptide with sequence C-QDIIPQLKKKYDVD, from the internal region of the protein sequence according to NP_000745.1, NP_009294.1.

Cross-Reactivity: Human

Format: Liquid

Grade: Serum-free

Description: This antibody is expected to recognise both reported isoforms (NP_000745.1 and NP_009294.1). Official Symbol: COMT

Specificity: This antibody is expected to recognise both reported isoforms (NP_000745.1 and NP_009294.1) Human 271 aa, 221 aa

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:64000. Western blot: Approx 28kDa band and faint 25kDa band observed in human testis lysates (calculated MW of 30.0kDa according to NP_000745.1 and 24.5kDa according to NP_009294.1). Recommended concentration: 0.03-0.1µg/ml. Enzyme immunoassay: Sandwich-type ELISA with increasing amount of recombinant COMTcaptured by a rabbit antibody. Recommended reporter concentration: 0.5-2ug/ml

Concentration: 100µg/200µl

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Signaling, Metabolism

Restrictions: For Research Use only

Images

Staining of human testis lysate (35µg protein in RIPA buffer) with A) EB07357 (0.03µg/ml) and B) ABIN185328 (1µg/ml). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Tunbridge, Harrison, Weinberger: "Catechol-o-methyltransferase, cognition, and psychosis: Val158Met and beyond." in: Biological psychiatry, Vol. 60, Issue 2, pp. 141-51, 2006 (PubMed).