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Catechol-O-Methyltransferase (COMT) (Internal Region) antibody

Details for Product No. ABIN185682, Supplier: Log in to see
Antigen
  • COMT
  • mb-comt
  • s-comt
  • comt
  • MGC154444
  • Comt1
  • D16Wsu103e
  • D330014B15Rik
Epitope
Internal Region
34
31
7
7
6
6
6
6
4
3
3
2
2
2
1
1
1
1
1
1
Reactivity
Human
162
39
37
7
3
3
3
3
2
2
1
1
Host
Goat
120
36
18
1
Clonality
Polyclonal
Conjugate
Un-conjugated
10
10
7
2
2
2
1
1
1
1
1
1
1
1
1
Methode Type
Peptide ELISA
Application
ELISA, Western Blotting (WB)
117
74
42
28
11
11
7
4
3
3
3
1
1
Supplier
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Immunogen Peptide with sequence C-QDIIPQLKKKYDVD, from the internal region of the protein sequence according to NP_000745.1, NP_009294.1.
Blocking Peptide Blocking peptide for this product available: ABIN369605
Specificity This antibody is expected to recognise both reported isoforms (NP_000745.1 and NP_009294.1)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Grade Serum-free
Alternative Name COMT (COMT Antibody Abstract)
Background Synonyms: COMT, catechol-O-methyltransferase
Gene ID 1312
NCBI Accession NP_000745, NP_009294
Research Area Signaling, Metabolism
Pathways
Application Notes ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx 28 kDa band and faint 25 kDa band observed in human testis lysates (calculated MW of 30.0 kDa according to NP_000745.1 and 24.5 kDa according to NP_009294.1). Recommended concentration: 0.03-0.1 µg/mL. Enzyme immunoassay: Sandwich-type ELISA with increasing amount of recombinant COMTcaptured by a rabbit antibody. Recommended reporter concentration: 0.5-2 µg/mL
Restrictions For Research Use only
Format Liquid
Concentration 100µg/200µl
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
 image for anti-Catechol-O-Methyltransferase (COMT) (Internal Region) antibody (ABIN185682) Staining of human testis lysate (35µg protein in RIPA buffer) with A) EB07357 (0.03µg...
Background publications Tunbridge, Harrison, Weinberger: "Catechol-o-methyltransferase, cognition, and psychosis: Val158Met and beyond." in: Biological psychiatry, Vol. 60, Issue 2, pp. 141-51, 2006 (PubMed).