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Catechol-O-Methyltransferase (COMT) (Internal Region) antibody

Details for Product No. ABIN185682
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Antigen
Synonyms Comt1, D16Wsu103e, D330014B15Rik, COMT, mb-comt, s-comt, comt, MGC154444
Epitope
Internal Region
(20), (12), (8), (4), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(79), (12), (10), (5), (1)
Host
Goat
(54), (19), (12)
Clonality
Polyclonal
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB)
(64), (44), (30), (7), (7), (6), (6), (4), (3), (1)
Pubmed 1 reference available
Quantity 100 µg
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Availability Available
Catalog No. ABIN185682
374.27 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen Peptide with sequence C-QDIIPQLKKKYDVD, from the internal region of the protein sequence according to NP_000745.1, NP_009294.1.
Specificity This antibody is expected to recognise both reported isoforms (NP_000745.1 and NP_009294.1)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Grade Serum-free
Alternative Name COMT
Background Synonyms: COMT, catechol-O-methyltransferase
Gene ID 1312
NCBI Accession NP_000745.1, NP_009294.1
Research Area Signaling, Metabolism
Application Notes ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx 28 kDa band and faint 25 kDa band observed in human testis lysates (calculated MW of 30.0 kDa according to NP_000745.1 and 24.5 kDa according to NP_009294.1). Recommended concentration: 0.03-0.1 µg/mL. Enzyme immunoassay: Sandwich-type ELISA with increasing amount of recombinant COMTcaptured by a rabbit antibody. Recommended reporter concentration: 0.5-2 µg/mL
Restrictions For Research Use only
Format Liquid
Concentration 100µg/200µl
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-Catechol-O-Methyltransferase (COMT) (Internal Region) antibody Staining of human testis lysate (35µg protein in RIPA buffer) with A) EB07357 (0.03µg/ml) and B) ABIN185328 (1µg/ml). Primary incubation was 1 hour. Detected by chemiluminescence.
General Tunbridge, Harrison, Weinberger: "Catechol-o-methyltransferase, cognition, and psychosis: Val158Met and beyond." in: Biological psychiatry, Vol. 60, Issue 2, pp. 141-51, 2006 (PubMed).

Hosts (54), (19), (12)
Reactivities (79), (12), (10), (5), (1)
Applications (64), (44), (30), (7), (7), (6), (6), (4), (3), (1)
Conjugates (1), (1), (1), (1), (1), (1)
Epitopes (20), (12), (8), (4), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN185682
374.27 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Validation Images
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