NHEJ1 antibody (C-Term)

Catalog No. ABIN185719

This Goat Polyclonal antibody specifically detects NHEJ1 in WB, ELISA and IHC. It exhibits reactivity toward Human.

Quick Overview for NHEJ1 antibody (C-Term) (ABIN185719)

Target: NHEJ1 (Nonhomologous End-Joining Factor 1 (NHEJ1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This NHEJ1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-NHEJ1 Antibody

Binding Specificity: C-Term

Purpose: XRCC4-like factor / NHEJ1

Sequence: QRPQLSKVKR KKPR

Cross-Reactivity: Dog, Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-QRPQLSKVKRKKPR, from the C Terminus of the protein sequence according to NP_079058.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Placenta shows nuclear staining in trophoblasts. Recommended concentration, 2.5-3.8 μg/mL.

Western Blot: Approx 38 kDa band observed in lysates of Human Skin, Testis and Thyroid gland (calculated size of 33.3 kDa according to NP_079058.1). The observed molecular weight corresponds to findings with antibodies from other sources. The 38 kDa band w

Peptide ELISA: antibody detection limit dilution 1:64000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for NHEJ1

Target: NHEJ1 (Nonhomologous End-Joining Factor 1 (NHEJ1))

Alternative Name: NHEJ1

Background: NHEJ1, XRCC4-like factor, nonhomologous end-joining factor 1, FLJ12610, XLF , Cernunnos

Gene ID: 79840

NCBI Accession: NP_079058

Pathways: DNA Damage Repair