Nonhomologous End-Joining Factor 1 (NHEJ1) antibody

Antigen: Nonhomologous End-Joining Factor 1 (NHEJ1)

Synonyms: XLF, FLJ12610, MGC130191, MGC130192, cernunnos, 1700029B21Rik, NHEJ1, MGC139115, nhej1

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Catalog no.: ABIN185719

Additional Information

Alternative name: XRCC4-like factor / NHEJ1

Gene ID: 79840

NCBI Acession: NP_079058.1

Immunogen: Peptide with sequence C-QRPQLSKVKRKKPR, from the C Terminus of the protein sequence according to NP_079058.1.

Cross-Reactivity: Human, Dog (Canine)

Format: Liquid

Grade: Serum-free

Description: Official Symbol: NHEJ1

Specificity: Human, Dog 299 aa

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:64000. Western blot: Approx 38kDa band observed in lysates of Human Skin, Testis and Thyroid gland (calculated size of 33.3kDa according to NP_079058.1). The observed molecular weight corresponds to findings with antibodies from other sources. The 38kDa band was successfully blocked by incubation with the immunizing peptide. Recommended concentration 0.1-0.3µg/ml. IHC: In paraffin embedded Human Placenta shows nuclear staining in trophoblasts. Recommended concentration, 2.5-3.8µg/ml.

Concentration: 100µg/200µl

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN185719 (0.1 ug/ml) staining of Human Thyroid lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Ahnesorg, Smith, Jackson: "XLF interacts with the XRCC4-DNA ligase IV complex to promote DNA nonhomologous end-joining." in: Cell, Vol. 124, Issue 2, pp. 301-13, 2006 (PubMed).