Roundabout, Axon Guidance Receptor, Homolog 1 (Drosophila) (ROBO1) antibody

Antigen: Roundabout, Axon Guidance Receptor, Homolog 1 (Drosophila) (ROBO1)

Synonyms: SAX3, DUTT1, FLJ21882, MGC131599, MGC133277, Gm310, AW494633, AW742721, ROBO1, robo1, sax3, dutt1, MGC122264, roundabout, roundabout-1

Clonality: Polyclonal

Host: Goat

Application: ELISA

Catalog no.: ABIN185724

Additional Information

Alternative name: Robo1 / DUTT1 (mouse)

Gene ID: 19876 (mouse), 58946 (rat)

Immunogen: C-QKARPAKKQKHQ

Format: Affinity-purified

Description: All names and symbols: Robo1, roundabout homolog 1 (Drosophila) , AW494633, AW742721, DUTT1 , roundabout homolog 1. Official Symbol: Robo1. RefSeq number(s): NP_062286.2

Specificity: Mouse, Rat, Dog 1612 aa

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:128,000. Western Blot: Preliminary experiments gave bands at approx 80kDa and 40kDa in lysates of Mouse Brain and Rat Brain after 0.03µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 176kDa according to NP_062286.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Publications

Publications: Andrews, Liapi, Plachez et al.: "Robo1 regulates the development of major axon tracts and interneuron migration in the forebrain." in: Development (Cambridge, England), Vol. 133, Issue 11, pp. 2243-52, 2006 (PubMed).