DVL3 antibody (AA 545-716)

Catalog No. ABIN1858676

Product Details anti-DVL3 Antibody

Target: DVL3 (Dishevelled, Dsh Homolog 3 (Drosophila) (DVL3))

Binding Specificity: AA 545-716

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DVL3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Purpose: Polyclonal Antibody to Dishevelled, Dsh Homolog 3 (DVL3)

Specificity: The antibody is a rabbit polyclonal antibody raised against DVL3. It has been selected for its ability to recognize DVL3 in immunohistochemical staining and western blotting.

Cross-Reactivity: Mouse

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Dishevelled, Dsh Homolog 3 (DVL3) corresdonding to His545~Met716 with N-terminal His and GST Tag

Isotype: IgG

Application Details

Application Notes:

Western blotting: 1-5 μg/mL Immunocytochemistry in formalin fixed cells: 5-20 μg/mL Immunohistochemistry in formalin fixed frozen section: 5-20 μg/mL Immunohistochemistry in paraffin section: 5-20 μg/mL Enzyme-linked Immunosorbent Assay: 0.05-2 μg/mL Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for DVL3

Target: DVL3 (Dishevelled, Dsh Homolog 3 (Drosophila) (DVL3))

Alternative Name: Dishevelled, Dsh Homolog 3 (DVL3 Products)

Synonyms: dsh3 antibody, dvl3 antibody, wu:fb75f04 antibody, wu:fi22h02 antibody, wu:fp61b09 antibody, dishevelled segment polarity protein 3 L homeolog antibody, dishevelled segment polarity protein 3 antibody, dishevelled segment polarity protein 3a antibody, dvl3.L antibody, dvl3 antibody, DVL3 antibody, Dvl3 antibody, dvl3a antibody

Background: Segment polarity protein dishevelled homolog DVL-3