MYL1 antibody (AA 3-142)

Catalog No. ABIN1859935

Product Details anti-MYL1 Antibody

Target: MYL1 (Myosin, Light Chain 1, Alkali, Skeletal, Fast (MYL1))

Binding Specificity: AA 3-142

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MYL1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Purpose: Polyclonal Antibody to Myosin Light Chain 1 (MYL1)

Specificity: The antibody is a rabbit polyclonal antibody raised against MYL1. It has been selected for its ability to recognize MYL1 in immunohistochemical staining and western blotting.

Cross-Reactivity: Mouse, Pig, Rat

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Myosin Light Chain 1 (MYL1) corresdonding to Phe3~Ala142 with N-terminal His Tag

Isotype: IgG

Application Details

Application Notes:

Western blotting: 0.5-2 μg/mL Immunocytochemistry in formalin fixed cells: 5-20 μg/mL Immunohistochemistry in formalin fixed frozen section: 5-20 μg/mL Immunohistochemistry in paraffin section: 5-20 μg/mL Enzyme-linked Immunosorbent Assay: 0.05-2 μg/mL Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for MYL1

Target: MYL1 (Myosin, Light Chain 1, Alkali, Skeletal, Fast (MYL1))

Alternative Name: Myosin Light Chain 1 (MYL1 Products)

Synonyms: MLC1F antibody, MLC3F antibody, alkali antibody, fast antibody, myosin antibody, skeletal antibody, AI325107 antibody, MLC1f antibody, MLC3f antibody, Mylf antibody, MLC3-f antibody, MLCf antibody, Mcl3 antibody, Mlc1 antibody, Mlc3 antibody, fj53a11 antibody, wu:fj53a11 antibody, zgc:65785 antibody, zgc:77231 antibody, MLC1f/3f antibody, XMLC1f antibody, XMLC3f antibody, myl1 antibody, myosin light chain 1 antibody, myosin, light chain 1, alkali; skeletal, fast antibody, myosin, light polypeptide 1 antibody, myosin, light chain 1 antibody, Myosin regulatory light chain 1 antibody, myosin light chain 1 L homeolog antibody, MYL1 antibody, Myl1 antibody, mlc-1 antibody, myl1 antibody, myl1.L antibody

Background: MLC1, MLC1F, MLC3F, Myosin light chain 1/3, skeletal muscle isoform, Myosin light chain A1/A2, Myosin Light Chain 1, Alkali, Fast Skeletal