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Antibodies
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anti-Cytokeratin 18 (KRT18) antibody
Cytokeratin 18 (KRT18) antibody
| Antigen |
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| Synonyms |
K18, CK18, Krt1-18, CYK18, KRT18, LOC100008885, MGC109143, krt18 |
| Clonality |
Monoclonal (DC-10) |
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Host
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Reactivity
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Human (141), Rat (Rattus) (57), Mouse (Murine) (56), Dog (Canine) (41), Rabbit (35), Cow (Bovine) (23), Pig (Porcine) (22), Chicken (20), Horse (Equine) (17), Mammalian (10), Hamster (5), None (4), Sheep (Ovine) (3), Bird (Avian) (2), Cat (Feline) (2), Monkey (1)
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Application
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 Alternatives Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
Western Blotting (WB) (83), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (76), Flow Cytometry (FACS) (67), Immunofluorescence (IF) (66), Immunohistochemistry (IHC) (33), Immunohistochemistry (Frozen Sections) (IHC (fro)) (28), Enzyme Immunoassay (EIA) (22), ELISA (20), Immunoprecipitation (IP) (18), Immunocytochemistry (ICC) (12), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (4), ELISA (Detection) (2), Immunoelectron Microscopy (IEM) (2)
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| Catalog no. |
ABIN187684 |
| Quantity |
0.1 mg |
| Price |
553.33 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 7 to 10 Business Days |
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Alternative name
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Cytokeratin 18
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Isotype
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IgG1 (Matching secondary antibodies)
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Clone
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DC-10
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Specificity
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Reacts with a 45 kDa human keratin 18. Species Reactivity: Human. Does not react with bovine, pig, sheep, dog, rabbit, rat or mouse, others not tested.
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Application Notes
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Immunocytochemistry Immunohistochemistry (Protease pre-treatment required for paraffin-embedded tissue sections) Western blot: Use at 1:1000 to stain MCF-7 cell line lysate. The optimal dilution for a specific application should be determined by the researcher.
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Buffer
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100 µg in PBS containing 15 mM sodium azide, pH 7.4. Concentration is 1 mg/ml.
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Storage
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Store undiluted antibody at 2 - 8 °C until expiry date. For long term, aliquot and store at -20 °C. Avoid freeze/thaw cycles.
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Research Area
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Signaling, Cytoskeleton, Extracellular Matrix
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Restrictions
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For Research Use only
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Alternatives for antigen "Cytokeratin 18 (KRT18)", type "Antibodies"
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Hosts
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Mouse (88), Rabbit (78), Guinea Pig (1), Sheep (1)
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Reactivities
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Human (141), Rat (Rattus) (57), Mouse (Murine) (56), Dog (Canine) (41), Rabbit (35), Cow (Bovine) (23), Pig (Porcine) (22), Chicken (20), Horse (Equine) (17), Mammalian (10), Hamster (5), None (4), Sheep (Ovine) (3), Bird (Avian) (2), Cat (Feline) (2), Monkey (1)
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Applications
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Western Blotting (WB) (83), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (76), Flow Cytometry (FACS) (67), Immunofluorescence (IF) (66), Immunohistochemistry (IHC) (33), Immunohistochemistry (Frozen Sections) (IHC (fro)) (28), Enzyme Immunoassay (EIA) (22), ELISA (20), Immunoprecipitation (IP) (18), Immunocytochemistry (ICC) (12), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (4), ELISA (Detection) (2), Immunoelectron Microscopy (IEM) (2)
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Conjugates
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FITC (13), Biotin (11), Alexa Fluor 488 (3), HRP (3), Alexa Flour 488 (2), Alexa Fluor 350 (2), Alexa Fluor 555 (2), Alexa Fluor 647 (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), Gold (2), PE (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy5.5 (2), PE,Cy7 (2), RBITC (2), Alexa Fluor 700 (1)
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Epitopes
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pSer33 (7), C-Term (5), Center (1), N-Term (1)
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