S1PR1 antibody (C-Term)

Catalog No. ABIN187786

This Rabbit Polyclonal antibody specifically detects S1PR1 in WB. It exhibits reactivity toward Human.

Quick Overview for S1PR1 antibody (C-Term) (ABIN187786)

Target: S1PR1 (Sphingosine-1-Phosphate Receptor 1 (S1PR1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This S1PR1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-S1PR1 Antibody

Binding Specificity: C-Term

Specificity: Reacts with human endothelial cell differentiation gene 1 (C-terminal), EDG-1. In Western blotting, detects an approximately 44 kDa band in RH7777 cells transfected with full length human EDG-1. For positive control, use RH7777 cells transfected with full length EDG-1. Species Reactivity: Human, other species not tested.

Sterility: 0.2 μm filtered

Immunogen: Synthetic peptide derived from the C-terminal of EDG-1 receptor.

Application Details

Application Notes: Western blotting: Use at 5-10 µg/ml using a human EDG-1 receptor transfected cell line. The optimal dilution for a specific application should be determined by the researcher.

Restrictions: For Research Use only

Handling

Buffer: Rabbit polyclonal antibody against human endothelial cell differentiation gene 1 (CT). 100 µl of 1 mg/ml purified antibody 0.2 µm sterile filtered in phosphate buffered saline with 0.08% sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Target Details for S1PR1

Target: S1PR1 (Sphingosine-1-Phosphate Receptor 1 (S1PR1))

Alternative Name: EDG-1 / S1P1

Background: EDG-1 belongs to a family of G-protein coupled receptors whose ligands are lysophospholipids. The ligand for EDG-1 is sphingosine-1-phosphate. There are 8 known members of the EDG receptor family and they are implicated in mediating growth related effects such as induction of cellular proliferation, alterations in differentiation and survival and suppression of apoptosis. They also evoke cellular effector functions that are dependent on cytoskeletal responses such as contraction, secretion, adhesion and chemotaxis. EDG receptors are developmentally regulated and differ in tissue distribution. The couple to multiple types of G proteins to signal through ras and MAP kinase, rho, phospholipase C and several protein tyrosine kinases. EDG-1 is expressed in cardiovascular, leukocyte containing and other tissues.

Pathways: Signaling Events mediated by VEGFR1 and VEGFR2