S1PR1 antibody (Extracellular, N-Term)

Catalog No. ABIN7887142

The Rabbit Polyclonal anti-S1PR1 antibody is suitable to detect S1PR1 in samples from Human. It has been validated for WB, IF, IHC, FACS, LCI and IC.

Quick Overview for S1PR1 antibody (Extracellular, N-Term) (ABIN7887142)

Target: S1PR1 (Sphingosine-1-Phosphate Receptor 1 (S1PR1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This S1PR1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FACS), Live Cell Imaging (LCI), Immunochromatography (IC)

Product Details anti-S1PR1 Antibody

Binding Specificity: AA 17-30, Extracellular, N-Term

Purpose: A Rabbit Polyclonal Antibody to Sphingosine 1-Phosphate Receptor 1

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse,rat - 13,14 amino acid residues identical

Characteristics: Anti-S1PR1 (EDG1) (extracellular) Antibody (ABIN7043771, ABIN7045270 and ABIN7045271) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize S1PR1 from human, rat, and mouse samples.

Purification: Affinity purified on immobilized antigen.

Immunogen: SDYVNYDIIVRHYN(C), corresponding to amino acid residues 17-30 of human S1PR1

Isotype: IgG

Application Details

Application Notes:

WB: 1:200

FC: 5 mg per reaction

ICC: 1:50

IHC: 1:100

IP: The optimal concentration should be determined by the user

Comment:

Cited Application: IHC

Negative Control: (ABIN7237267)

Blocking Peptide: (ABIN7237267)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for S1PR1

Target: S1PR1 (Sphingosine-1-Phosphate Receptor 1 (S1PR1))

Alternative Name: S1PR1

Background:

Synonyms: Sphingosine 1-phosphate receptor 1, S1P1, Endothelial differentiation G-protein coupled receptor 1, CD363, CHEDG1

Description: Lysophospholipids constitute a group of important lipid mediators, lysophosphatidic acid (LPA) a glycerolysophospholipid and sphingosine 1-phosphate (S1P), a lysosphingolipid. Sphingosine 1-phosphate is a bioactive lipid derived from metabolism of sphingomyelin1. Sphingosine 1-phosphate is implicated in the regulation of many cellular functions including proliferation, apoptosis, survival, adhesion, differentiation, and migration2. Sphingosine 1-phosphate exerts its activity through five distinct G-protein-coupled receptors, (also named endothelial differentiation gene receptors - EDG), S1PR1 (EDG-1), S1PR2 (EDG-5), S1PR3 (EDG-3), S1PR4 (EDG-6), and S1PR5 (EDG-8)3.Sphingosine 1-phosphate receptor 1 (S1PR1) is ubiquitously expressed. S1PR1 mRNA is detected in the brain, heart, spleen, liver, lung, thymus, kidney, skeletal muscle and lymphoid tissues4. Knockout of the gene encoding S1PR1 is lethal and therefore makes in vivo studies difficult5.

Gene ID: 1901

NCBI Accession: NM_001400

UniProt: P21453

Pathways: Signaling Events mediated by VEGFR1 and VEGFR2