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Src antibody (N-Term)

There are 5+ publications for this product available. The Rabbit Polyclonal anti-Src antibody is suitable to detect Src in samples from Human. It has been validated for WB, IF, IHC (p) and FACS.

SRC Antibody (N-term) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram). (Src antibody (N-Term))

FACS

Confocal immunofluorescent analysis of SRC Antibody (N-term) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). (Src antibody (N-Term))

Confocal immunofluorescent analysis of SRC Antibody (N-term) with A431 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). (Src antibody (N-Term))

SRC Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human small intestine tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. (Src antibody (N-Term))

IHC (p)

SRC Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. (Src antibody (N-Term))

IHC (p)

6 images

(5 references)

Catalog No. ABIN1881833

$451.00

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Shipping to: United States

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Quick Overview for Src antibody (N-Term) (ABIN1881833)

Target

See all Src Antibodies

Src (Proto-oncogene tyrosine-protein kinase Src (Src))

Reactivity

Human

Host

Rabbit

Clonality

Polyclonal

Conjugate

This Src antibody is un-conjugated

Application

Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)

Clone

RB41141

Discover our top product Src Primary Antibody

Binding Specificity
34

28

25

20

17

15

13

11

10

8

7

6

4

4

4

4

3

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 24-52, N-Term

Predicted Reactivity

M

Purification

This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen

This SRC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 24-52 amino acids from the N-terminal region of human SRC.

Isotype

IgG
anti-Proto-oncogene tyrosine-protein kinase Src (Src) antibody
Src Reactivity: Human WB, IHC, ELISA, FACS Host: Mouse Monoclonal 1F11 unconjugated

anti-Proto-oncogene tyrosine-protein kinase Src (Src) antibody
Src Reactivity: Human, Mouse WB, IF Host: Mouse Monoclonal 17AT28 unconjugated

anti-Proto-oncogene tyrosine-protein kinase Src (Src) (pTyr530) antibody
Src Reactivity: Human, Mouse, Rat WB, IHC, ELISA, IF, ICC Host: Rabbit Polyclonal unconjugated

anti-Proto-oncogene tyrosine-protein kinase Src (Src) antibody
KD Validated Src Reactivity: Human WB, IF (p), FACS Host: Rabbit Monoclonal 23GB985 unconjugated Recombinant Antibody

anti-Proto-oncogene tyrosine-protein kinase Src (Src) antibody
KO Validated Src Reactivity: Human WB, IF, IP Host: Rabbit Monoclonal unconjugated

anti-Proto-oncogene tyrosine-protein kinase Src (Src) (AA 397-424) antibody
Src Reactivity: Human WB, IHC, ELISA, IF Host: Rabbit Polyclonal unconjugated

anti-Proto-oncogene tyrosine-protein kinase Src (Src) (AA 24-52) antibody
Src Reactivity: Human WB, IHC, ELISA, IF, FACS Host: Rabbit Polyclonal unconjugated

anti-Proto-oncogene tyrosine-protein kinase Src (Src) (AA 1-90) antibody
Src Reactivity: Human WB, ELISA, IF, IHC (p), PLA Host: Mouse Monoclonal 1B9 unconjugated

anti-Proto-oncogene tyrosine-protein kinase Src (Src) (pTyr419) antibody
Src Reactivity: Human, Mouse, Rat WB, IHC, ELISA, IF, ICC Host: Rabbit Polyclonal unconjugated

anti-Proto-oncogene tyrosine-protein kinase Src (Src) (pTyr529) antibody
Src Reactivity: Human WB, IHC, ELISA, IF Host: Rabbit Polyclonal unconjugated

Application Notes

IF: 1:50. IF: 1:50. WB: 1:2000. IHC-P: 1:50. IHC-P: 1:50. FC: 1:50

Restrictions

For Research Use only
Format

Liquid

Buffer

Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

4 °C,-20 °C

Expiry Date

6 months
Kieslinger, Hiechinger, Dobreva, Consalez, Grosschedl: "Early B cell factor 2 regulates hematopoietic stem cell homeostasis in a cell-nonautonomous manner." in: Cell stem cell, Vol. 7, Issue 4, pp. 496-507, (2010) (PubMed).

Pradhan, Alrehani, Patel, Khatlani, Vijayan: "Cross-talk between serine/threonine protein phosphatase 2A and protein tyrosine phosphatase 1B regulates Src activation and adhesion of integrin αIIbβ3 to fibrinogen." in: The Journal of biological chemistry, Vol. 285, Issue 38, pp. 29059-68, (2010) (PubMed).

Dergai, Tsyba, Dergai, Zlatskii, Skrypkina, Kovalenko, Rynditch: "Microexon-based regulation of ITSN1 and Src SH3 domains specificity relies on introduction of charged amino acids into the interaction interface." in: Biochemical and biophysical research communications, Vol. 399, Issue 2, pp. 307-12, (2010) (PubMed).

Liu, Qiu, Li, Chen, Cao et al.: "Human phosphatidylethanolamine-binding protein 4 promotes transactivation of estrogen receptor alpha (ERalpha) in human cancer cells by inhibiting proteasome-dependent ERalpha degradation via ..." in: The Journal of biological chemistry, Vol. 285, Issue 29, pp. 21934-42, (2010) (PubMed).

Pene-Dumitrescu, Smithgall: "Expression of a Src family kinase in chronic myelogenous leukemia cells induces resistance to imatinib in a kinase-dependent manner." in: The Journal of biological chemistry, Vol. 285, Issue 28, pp. 21446-57, (2010) (PubMed).
Target

Src (Proto-oncogene tyrosine-protein kinase Src (Src))

Alternative Name

SRC

Background

This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene.

Molecular Weight

59835

NCBI Accession

NP_005408, NP_938033

UniProt

P12931

Pathways

JAK-STAT Signaling, Neurotrophin Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Cellular Response to Molecule of Bacterial Origin, Cell-Cell Junction Organization, Regulation of Carbohydrate Metabolic Process, Autophagy, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling, Platelet-derived growth Factor Receptor Signaling, Thromboxane A2 Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling