TRMT12 antibody (N-Term)

Catalog No. ABIN1888127

Product Details anti-TRMT12 Antibody

Target: TRMT12 (tRNA Methyltransferase 12 Homolog (TRMT12))

Binding Specificity: N-Term

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TRMT12 antibody is un-conjugated

Application: ELISA, Western Blotting (WB)

Cross-Reactivity (Details): TYW2 antibody is predicted to not cross-react with other TYW protein family members. At least two isoforms of TYW2 are known to exist, this antibody will detect both isoforms

Purification: Affinity chromatography purified via peptide column

Immunogen: 19 amino acid peptide near the amino terminus of human TYW2.

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid freezing and thawing repeatly.

Storage: 4 °C/-20 °C

Storage Comment: Store at 4 °C for short term use.Store at -20 °C for long term preservation.

Target Details for TRMT12

Target: TRMT12 (tRNA Methyltransferase 12 Homolog (TRMT12))

Alternative Name: TYW2 (TRMT12 Products)

Synonyms: 4632406N01Rik antibody, AI449275 antibody, Tyw2 antibody, TRM12 antibody, TYW2 antibody, RGD1307127 antibody, trmt12 antibody, tRNA methyltranferase 12 antibody, tRNA methyltransferase 12 homolog antibody, tRNA methyltransferase 12 antibody, tRNA methyltransferase 12 homolog (S. cerevisiae) antibody, Trmt12 antibody, TRMT12 antibody, trmt12 antibody

Background: TYW2, also known as TRM12, is an enzyme that participates in the wybutosine-tRNA (Phe) biosynthesis pathway.Wybutosine (yW) is a hypermodified guanosine at the 3-prime position adjacent to the anticodon of phenylalanine tRNA that stabilizes codon-anticodon interactions during decoding on the ribosome.TYW2 is involved in a multistep enzymatic reaction that stabilizes codon-anticodon base-pairing during the ribosomal decoding process, thereby ensuring correct translation.TYW2 was found to be amplified in 7 of 8 breast cancers cell lines analyzed, and qPCR analysis of 30 breast tumors showed overexpression of TYW2 mRNA of over 2-fold in 26, suggesting that TYW2 may play a role in breast cancer.
Synonyms: TYW2, Radical S-adenosyl methionine and flavodoxin domain-containing protein 2, tRNA wybutosine-synthesizing protein 2 homolog, TRM12, TRMT12

UniProt: Q53H54