Isocitrate Dehydrogenase (IDH) antibody

Antigen: Isocitrate Dehydrogenase (IDH)

Synonyms: CT22171, IDH, IDH-NADP, l(3)L3852, DmelCG7176, CG7176, F20D21.16, F20D21_16, ICDH, ISOCITRATE DEHYDROGENASE, icd, F6P23.14, F6P23_14, IDH2, ISOCITRATE DEHYDROGENASE SUBUNIT 2, NAD+ DEPENDENT ISOCITRATE DEHYDROGENASE SUBUNIT 2

Clonality: Polyclonal

Host: Rabbit

Reactivity: Arabidopsis thaliana, Pea (Pisum sativum), Potato (Solanum tuberosum), Maize/Corn (Zea mays)

Application: Western Blotting (WB)

Catalog no.: ABIN190717

Additional Information

Swiss-Prot: P93032

Immunogen: KLH-conjugated peptide 1 and peptide 2 conserved in all higher plantsmitochondrial, NAD dependent isocitrate dehydrogenase subunits includingArabidopsis thaliana IDH-I Q8LFCO and IDH-II P93032

Format: Lyophilized

Description: Plant NADH dependent isocitrate dehydrogenase enzyme is located inmitochondrial matrix. This enzyme is classified as an oxidoreductase and itsfunction is to catalyze a reaction in the citric acid cycle, specifically the sequentialdehydrogenation and decarboxylation of isocitrate to form a-ketoglutarate. Itremoves hydrogens from its substrate, isocitrate. In addition to this process, itfunctions as a decarboxylase, removing a CO2 from the six-carbon substrate toform a five-carbon

Specificity: Predicted reactivity: dicots including Brassica napus, Vitis vinifera, monocots including Oryza sativa,Zea mays. Not reactive in: Chlamydomonas reinhardtii.

Synonyms: CT22171, IDH, IDH-NADP, l(3)L3852, DmelCG7176, CG7176, F20D21.16, F20D21_16, ICDH, ISOCITRATE DEHYDROGENASE, icd, F6P23.14, F6P23_14, IDH2, ISOCITRATE DEHYDROGENASE SUBUNIT 2, NAD+ DEPENDENT ISOCITRATE DEHYDROGENASE SUBUNIT 2

Application Details

Application Notes: Recommended Dilution 1 : 5 000 with standard ECL (WB). Additional Information: Peptide used to elicit this antibody is not conserved in NADPH dependentanzymes, partially conserved across eukaryotic Idh subunits. Some conservationacross bacterial which contain the NAD-dependent form of Idh (as opposed to theNADP-dependent form).

Buffer: Affinity purified serum in PBS pH 7.4

Storage: store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior toopening them to avoid any losses that might occur from lyophilized materialadhering to the cap or sides of the tubes.

Research Area: Plant/Algal Physiology

Restrictions: For Research Use only

Images

Figure description: 1: Total extract A. thaliana (20 g protein) 2: Fraction enriched with mitochondria A. thaliana 3: Pure mitochondria A. thaliana 4: Pure mitochondria P. satisum 5: Pure mitochondria S. tuberosum Description of experimental conditions: After SDS-PAGE gel electrophoresis samples have been transferred to nitrocellulose membrane. Blocking has been done in 5% milk powder in TBS followed by incubation with primary antibodies for 1 hour and 30 minutes in RT After incubation with secondary antibodies reaction has been developed using ECL reagent (GE Healthcare) * Band detected at ca. 90 kDa is suspected to be a dimmer of Idh, since this band is depleted upon peptide competition experiment.

Publications

Publications: Szal, Jastrz?bska, Kulka et al.: "Influence of mitochondrial genome rearrangement on cucumber leaf carbon and nitrogen metabolism." in: Planta, Vol. 232, Issue 6, pp. 1371-82, 2010 (PubMed).