Peptidylprolyl Isomerase D (PPID) antibody

Antigen: Peptidylprolyl Isomerase D (PPID)

Synonyms: CYPD, CYP-40, MGC33096, CYP3, Cyp-D, FLJ90798, MGC117207, Ppif, Ppidl, 4930564J03Rik, CypD, CyP-D, CyP-F, PPIase, AW457192, MGC137782, MGC93084, Cyp-40, MGC93768, CYCLOPHILIN 40, CYCLOPHILIN-40, CYP40, F19G14.21, F19G14_21, SQN, SQUINT, zgc:86711, PPID

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN190756

Additional Information

Alternative name: PPID / CyP-40

Gene ID: 5481, 67738 (mouse), 361967 (rat)

Immunogen: C-DENFHYKHDREG

Format: Affinity-purified

Description: All names and symbols: CYP-40, MGC33096, peptidylprolyl isomerase D (cyclophilin D) , 40 kDa peptidyl-prolyl cis-trans isomerase D, PPIase, cyclophilin 40, cyclophilin D, cyclophilin-related protein, peptidylprolyl isomerase D, rotamase. Official Symbol: PPID. RefSeq number(s): NP_005029.1

Specificity: Human, Mouse, Rat, Dog

Synonyms: CYPD, CYP-40, MGC33096, CYP3, Cyp-D, FLJ90798, MGC117207, Ppif, Ppidl, 4930564J03Rik, CypD, CyP-D, CyP-F, PPIase, AW457192, MGC137782, MGC93084, Cyp-40, MGC93768, CYCLOPHILIN 40, CYCLOPHILIN-40, CYP40, F19G14.21, F19G14_21, SQN, SQUINT, zgc:86711, PPID

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:64,000. Western Blot: Approx 40kDa band observed in Human Brain (Frontal Cortex) lysates (calculated MW of 40.8kDa according to NP_005029.1). In transfected HEK293 transiently expressing CyP-40 a band of approx. 45kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.1-0.3µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN190756 (0.1µg/ml) staining of Human Brain (Frontal Cortex) lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

HEK293 overexpressing CyP-40 (RC206039) and probed with ABIN190756 (mock transfection in first lane), tested by Origene.

Publications

Publications: Forte, Gold, Marracci et al.: "Cyclophilin D inactivation protects axons in experimental autoimmune encephalomyelitis, an animal model of multiple sclerosis." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, Issue 18, pp. 7558-63, 2007 (PubMed).