Interleukin Enhancer Binding Factor 2, 45kDa (ILF2) antibody

Antigen: Interleukin Enhancer Binding Factor 2, 45kDa (ILF2)

Synonyms: NF45, MGC8391, PRO3063, ilf2, MGC53292, nf45, pro3063, MGC75701, ILF2, MGC134524, DKFZp469J1839

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB), Immunofluorescence (IF)

Catalog no.: ABIN190786

Additional Information

Alternative name: ILF2 / NF45

Gene ID: 3608, 67781 (mouse), 310612 (rat)

Immunogen: C-EKAYEKPPEKKEG

Format: Affinity-purified

Description: All names and symbols: ILF2, NF45, interleukin enhancer binding factor 2, 45kDa , MGC8391, PRO3063 , interleukin enhancer binding factor 2, nuclear factor of activated T-cells, 45-kDa. Official Symbol: ILF2. RefSeq number(s): NP_004506.2

Specificity: Human, Mouse, Rat, Dog

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:4,000. Western Blot: Approx 45kDa band observed in Human Tonsil and Thymus lysates (calculated MW of 43.1kDa according to NP_004506.2). Recommended concentration: 0.3-1µg/ml. Immunofluorescence: Paraformaldehyde-fixed stable knock-down cell line (second half) shows merely background compared to the wildtype cell line (left half). Data kindly provided by Dr. A Antes, UMDNJ, Newark, USA Recommended concentration, 2-3µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN190786 (0.3µg/ml) staining of Human Tonsil lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

ABIN190786 (2.5µg/ml) staining of parental and stable knock-down cell line (fixed by 4% formaldehyse, permealized by Triton X100). Primary incubation was 1.5 hour. Detected by fluorescence.

Publications

Publications: Wang, Rao, Chu et al.: "A protein interaction network for pluripotency of embryonic stem cells." in: Nature, Vol. 444, Issue 7117, pp. 364-8, 2006 (PubMed).