ILF2 antibody (Interleukin Enhancer Binding Factor 2, 45kDa) (C-Term)

Details for Product anti-ILF2 Antibody No. ABIN2776419, Supplier: Log in to see
Antigen
  • nf45
  • pro3063
  • ILF2
  • wu:fb34h01
  • zgc:73084
  • zgc:77276
  • NF45
  • PRO3063
  • 6230405A16Rik
  • TEG-267
  • Tex261
  • Tex267
  • interleukin enhancer binding factor 2
  • interleukin enhancer binding factor 2 L homeolog
  • ilf2
  • ILF2
  • ilf2.L
  • Ilf2
Epitope
C-Term
15
14
13
9
6
4
2
2
2
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus), Zebrafish (Danio rerio)
95
21
18
15
7
7
6
5
4
4
4
2
2
2
Host
Rabbit
57
22
16
Clonality
Polyclonal
Conjugate
This ILF2 antibody is un-conjugated
9
7
7
3
3
3
Application
Immunohistochemistry (IHC), Western Blotting (WB)
86
55
34
21
14
5
4
2
2
2
1
Options
Supplier
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Immunogen The immunogen is a synthetic peptide directed towards the C terminal region of human ILF2
Sequence HGGFRKILGQ EGDASYLASE ISTWDGVIVT PSEKAYEKPP EKKEGEEEEE
Predicted Reactivity Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 93%
Characteristics This is a rabbit polyclonal antibody against ILF2. It was validated on Western Blot and immunohistochemistry.
Purification Protein A purified
Alternative Name ILF2 (ILF2 Antibody Abstract)
Background Nuclear factor of activated T-cells (NFAT) is a transcription factor required for T-cell expression of the interleukin 2 gene. NFAT binds to a sequence in the interleukin 2 gene enhancer known as the antigen receptor response element 2. In addition, NFAT can bind RNA and is an essential component for encapsidation and protein priming of hepatitis B viral polymerase. NFAT is a heterodimer of 45 kDa and 90 kDa proteins, the smaller of which is the product of the ILF2 gene. The encoded protein binds strongly to the 90 kDa protein and stimulates its ability to enhance gene expression.
Alias Symbols: NF45, PRO3063
Protein Interaction Partner: CCNDBP1, HUWE1, UBC, EEF1G, AURKA, SUMO2, SUMO3, STAU1, IVNS1ABP, RPA1, SMURF2, RPA3, RPA2, ERG, EZH2, BMI1, SUZ12, EED, rev, HNRNPU, FKBP3, DHX9, DDX1, ABCF1, C14orf166, RTCB, DIMT1, NELFB, EDC4, IGF2BP3, EIF2B2, EIF2B3, YBX3, RFC4, PTBP1, YBX1, NMT1, MR
Protein Size: 390
Molecular Weight 43 kDa
Gene ID 3608
NCBI Accession NM_004515, NP_004506
UniProt Q12905
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 390 AA

Restrictions For Research Use only
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Supplier Images
Western Blotting (WB) image for anti-Interleukin Enhancer Binding Factor 2, 45kDa (ILF2) (C-Term) antibody (ABIN2776419) WB Suggested Anti-ILF2 Antibody Titration: 1.25ug/ml
ELISA Titer: 1:...
Western Blotting (WB) image for anti-Interleukin Enhancer Binding Factor 2, 45kDa (ILF2) (C-Term) antibody (ABIN2776419) Sample Type: MCF-7 WT a whole cell lysates (110UG)
Primary Dilution:
Immunohistochemistry (IHC) image for anti-Interleukin Enhancer Binding Factor 2, 45kDa (ILF2) (C-Term) antibody (ABIN2776419) anti-Interleukin Enhancer Binding Factor 2, 45kDa (ILF2) (C-Term) antibody (Image 3)
Western Blotting (WB) image for anti-Interleukin Enhancer Binding Factor 2, 45kDa (ILF2) (C-Term) antibody (ABIN2776419) Host: Rabbit
Target Name: ILF2
Sample Tissue: Jurkat
Lane A: Pri...
Western Blotting (WB) image for anti-Interleukin Enhancer Binding Factor 2, 45kDa (ILF2) (C-Term) antibody (ABIN2776419) WB Suggested Anti-ILF2
Antibody Titration: 1.25 µg/mL ELISA Titer: 1:62500
Immunohistochemistry (IHC) image for anti-Interleukin Enhancer Binding Factor 2, 45kDa (ILF2) (C-Term) antibody (ABIN2776419) Human kidney
Product cited in: Yamauchi, Nakamura, Hiramoto, Yuri, Yokota, Naitou, Takeuchi, Yamanaka, Kita, Nakahara, Kinoyama, Matsuhisa, Kaneko, Koutoku, Sasamata, Kobori, Katou, Tawara, Kawabata, Furuichi: "Sepantronium bromide (YM155) induces disruption of the ILF3/p54(nrb) complex, which is required for survivin expression." in: Biochemical and biophysical research communications, Vol. 425, Issue 4, pp. 711-6, 2012 (PubMed).

Jiang, Zhou, Moxley, Jarrett: "Purification and identification of positive regulators binding to a novel element in the c-Jun promoter." in: Biochemistry, Vol. 47, Issue 35, pp. 9318-34, 2008 (PubMed).

Background publications Shin, Kim, Cho, Lee, Rho: "Host cell proteins binding to the encapsidation signal epsilon in hepatitis B virus RNA." in: Archives of virology, Vol. 147, Issue 3, pp. 471-91, 2002 (PubMed).

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