CLIC4 antibody (N-Term)

Catalog No. ABIN190810

This Goat Polyclonal antibody specifically detects CLIC4 in WB, IHC, ELISA and IF. It exhibits reactivity toward Human and Mouse.

Quick Overview for CLIC4 antibody (N-Term) (ABIN190810)

Target: CLIC4 (Chloride Intracellular Channel 4 (CLIC4))

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This CLIC4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Grade: Verified

Product Details anti-CLIC4 Antibody

Binding Specificity: N-Term

Purpose: CLIC4

Sequence: NGLKEEDKEP LIE

Cross-Reactivity: Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence NGLKEEDKEPLIE-C, from the N Terminus of the protein sequence according to NP_039234.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Heart. Recommended concentration: 5 μg/mL.

Western Blot: Approx 30 kDa band observed in Human Kidney and Placenta lysates (calculated MW of 28.8 kDa according to NP_039234.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Edwards et al, Am J

Peptide ELISA: antibody detection limit dilution 1:64000.

Comment:

Immunofluorescence: Min6 cells transiently expressing mouse Clic4 shows cytoplasmic staining of two cells. Recommended concentration, 5μg/ml with overnight incubation.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for CLIC4

Target: CLIC4 (Chloride Intracellular Channel 4 (CLIC4))

Alternative Name: CLIC4

Background: CLIC4, chloride intracellular channel 4, CLIC4L, DKFZP566G223, FLJ38640, H1, huH1, p64H1 , chloride intracellular channel 4 like

Gene ID: 25932, 29876, 83718

NCBI Accession: NP_039234