PLIN1 antibody (C-Term)

Catalog No. ABIN190817

Product Details anti-PLIN1 Antibody

Target: PLIN1 (Perilipin 1 (PLIN1))

Binding Specificity: C-Term

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This PLIN1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purpose: Perilipin 1 (C Terminus)

Sequence: EPILGRAQYS QLRKK

Cross-Reactivity: Dog, Human, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-EPILGRAQYSQLRKK, from the C Terminus of the protein sequence according to NP_002657.2.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 55 kDa band observed in Human Breast and Breast cancer lysates, and in lysates of cell line Jurkat (calculated MW of 56.0 kDa according to NP_002657.3). Recommended concentration: 0.5-2 μg/mL. Primary incubation 1 hour at room temperatu

Peptide ELISA: antibody detection limit dilution 1:64000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-PLIN1 antibody (ABIN190817)

Mottillo, Zhang, Yang, Zhou, Granneman: "Genetically-encoded sensors to detect fatty acid production and trafficking." in: Molecular metabolism, Vol. 29, pp. 55-64, (2020) (PubMed).

Ramseyer, Kimler, Granneman: "Vacuolar protein sorting 13C is a novel lipid droplet protein that inhibits lipolysis in brown adipocytes." in: Molecular metabolism, Vol. 7, pp. 57-70, (2019) (PubMed).

Mottillo, Paul, Moore, Granneman: "Use of fluorescence microscopy to probe intracellular lipolysis." in: Methods in enzymology, Vol. 538, pp. 263-78, (2014) (PubMed).

Target Details for PLIN1

Target: PLIN1 (Perilipin 1 (PLIN1))

Alternative Name: PLIN (PLIN1 Products)

Synonyms: FPLD4 antibody, PERI antibody, PLIN antibody, PERIA antibody, Plin antibody, 6030432J05Rik antibody, Peri antibody, peri antibody, plin antibody, perilipin antibody, LOC692833 antibody, perilipin 1 antibody, perilipin antibody, perilipin 1 L homeolog antibody, PLIN1 antibody, Plin1 antibody, plin1 antibody, LOC692833 antibody, plin1.L antibody

Background: Plin, perilipin, PERI, lipid droplet-associated protein, PLIN1, Perilipin1, Perilipin 1

Gene ID: 5346, 25629

NCBI Accession: NP_002657

Pathways: Lipid Metabolism