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Perilipin 1 (PLIN1) (C-Term) antibody

Details for Product No. ABIN190817, Supplier: Log in to see
Antigen
  • PLIN
  • peri
  • plin
  • perilipin
  • LOC692833
  • FPLD4
  • PERI
  • 6030432J05Rik
  • Peri
  • Plin
  • PERIA
Epitope
C-Term
39
13
10
8
7
3
3
2
2
1
1
1
1
1
Reactivity
Human
82
43
40
9
9
8
2
2
1
1
1
Host
Goat
45
37
7
6
3
Clonality
Polyclonal
Conjugate
Un-conjugated
4
4
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Methode Type
Peptide ELISA
Application
ELISA, Western Blotting (WB)
84
37
20
13
11
10
6
2
1
Supplier
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Immunogen Peptide with sequence C-EPILGRAQYSQLRKK, from the C Terminus of the protein sequence according to NP_002657.2.
Blocking Peptide Blocking peptide for this product available: ABIN369038
Cross-Reactivity Rat (Rattus), Dog (Canine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name Perilipin (PLIN1 Antibody Abstract)
Background Synonyms: Plin, perilipin, PERI, lipid droplet-associated protein
Gene ID 5346, 25629
NCBI Accession NP_002657
Pathways
Application Notes ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 55-60 kDa band observed in Human Adipose lysates (calculated MW of 56.0 kDa according to NP_002657.2). Recommended concentration: 0.01-0.03 µg/mL. Immunofluorescence: Small lipid droplets preferentially stained in the periphery of differentiated 3T3-L1 adipocytes. Recommended concentration 1 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
 image for anti-Perilipin 1 (PLIN1) (C-Term) antibody (ABIN190817) ABIN190817 (0.01µg/ml) staining of Human Adipose lysate (35µg protein in RIPA buffer)...
 image for anti-Perilipin 1 (PLIN1) (C-Term) antibody (ABIN190817) ABIN190817 staining of differentiated 3T3-L1 adipocytes. Data kindly provided by Prof...
Background publications Wolins, Brasaemle, Bickel: "A proposed model of fat packaging by exchangeable lipid droplet proteins." in: FEBS letters, Vol. 580, Issue 23, pp. 5484-91, 2006 (PubMed).