Perilipin 1 (PLIN1) (C-Term) antibody

Antigen: Perilipin 1 (PLIN1)

Synonyms: PERI, PLIN, PERIA, Plin1, Peri, Plin, 6030432J05Rik, PLIN1, MGC138955, peri, plin, perilipin, LOC692833

Epitope: C-Term

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB), Immunofluorescence (IF)

Catalog no.: ABIN190817

Additional Information

Alternative name: Perilipin (C Terminus)

Gene ID: 5346, 25629 (rat)

Immunogen: C-EPILGRAQYSQLRKK

Format: Affinity-purified

Description: All names and symbols: Plin, perilipin, PERI, lipid droplet-associated protein. Official Symbol: PLIN. RefSeq number(s): NP_002657.2

Specificity: Human, Rat, Dog

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx 55-60kDa band observed in Human Adipose lysates (calculated MW of 56.0kDa according to NP_002657.2). Recommended concentration: 0.01-0.03 µg/ml. Immunofluorescence: Small lipid droplets preferentially stained in the periphery of differentiated 3T3-L1 adipocytes. Recommended concentration 1ug/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN190817 (0.01µg/ml) staining of Human Adipose lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

ABIN190817 staining of differentiated 3T3-L1 adipocytes. Data kindly provided by Prof. J. Granneman, Detroit, USA

Publications

Publications: Wolins, Brasaemle, Bickel: "A proposed model of fat packaging by exchangeable lipid droplet proteins." in: FEBS letters, Vol. 580, Issue 23, pp. 5484-91, 2006 (PubMed).