Carabin antibody (Internal Region)

Catalog No. ABIN190840

This Goat Polyclonal antibody specifically detects Carabin in WB, ELISA and FACS. It exhibits reactivity toward Human and Mouse.

Quick Overview for Carabin antibody (Internal Region) (ABIN190840)

Target: Carabin (TBC1D10C) (TBC1 Domain Family, Member 10C (TBC1D10C))

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This Carabin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Grade: Verified

Product Details anti-Carabin Antibody

Binding Specificity: Internal Region

Purpose: Carabin / TBC1D10C

Sequence: PRRKPQTRGK TFH

Cross-Reactivity: Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-PRRKPQTRGKTFH, from the internal region of the protein sequence according to NP_940919.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 48-50 kDa band observed in lysates of cell line Jurkat and in Mouse Thymus lysates and approx 50 kDa in Peripheral Blood Mononuclear (PBM) and in Human Spleen lysates (calculated MW of 49.7Da according to Human NP_940919.1 and 49.9 kDa

Peptide ELISA: antibody detection limit dilution 1:128000.

Comment:

Flow Cytometry: Flow cytometric analysis of Jurkat cells. Recommended concentration: 10ug/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for Carabin

Target: Carabin (TBC1D10C) (TBC1 Domain Family, Member 10C (TBC1D10C))

Alternative Name: TBC1D10C

Background: TBC1D10C, carabin, TBC1 domain family, member 10C, FLJ00332, MGC46488

Gene ID: 374403, 108995

NCBI Accession: NP_940919