Neuregulin 3 (NRG3) antibody

Antigen: Neuregulin 3 (NRG3)

Synonyms: HRG3, pro-NRG3, ska, NRG3, RGD1559678

Clonality: Polyclonal

Host: Goat

Reactivity: Human, Mouse (Murine), Rat (Rattus)

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN190905

Additional Information

Alternative name: Neuregulin 3

Gene ID: 10718, 18183 (mouse)

Immunogen: C-RNEIQRDSALTK

Format: Affinity-purified

Description: All names and symbols: neuregulin 3, HGNC:7999, NRG3, HRG3, pro-NRG3. Official Symbol: NRG3. RefSeq number(s): NP_001010848.2

Specificity: Human, Mouse, Rat, Dog

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:64,000. Western Blot: Approx 75kDa band observed in Human Brain (Cerebellum) lysates and in Mouse and Rat Brain lysates (calculated MW of 75.0kDa according to NP_001010848.2). Recommended concentration: 0.1-0.3µg/ml. An additional band of unknown identity was also consistently observed at 37kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN190905 (0.3µg/ml) staining of Human Brain (Cerebellum) lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Hijazi, Young, Dougherty et al.: "NRG-3 in human breast cancers: activation of multiple erbB family proteins." in: International journal of oncology, Vol. 13, Issue 5, pp. 1061-7, 1998 (PubMed).