Allograft Inflammatory Factor 1 (AIF1) (isoform 1 and 3) antibody

Antigen: Allograft Inflammatory Factor 1 (AIF1)

Synonyms: IBA1, AIF-1, IRT-1, G1, Iba1, AI607846, D17H6S50E, iba1, Bart1, mrf-1, fa04b11, wu:fa04b11, AIF1, AIF, aif1

Epitope: isoform 1 and 3

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN190906

Additional Information

Alternative name: AIF1 / IBA1 (isoforms 1 + 3)

Gene ID: 199

Immunogen: C-YEEKAREKEKP

Format: Affinity-purified

Description: All names and symbols: AIF1, IBA1, allograft inflammatory factor 1, DASS-82G15.2, AIF-1, IRT-1, Em:AF129756.17, interferon gamma responsive transcript, ionized calcium-binding adapter molecule. Official Symbol: AIF1. RefSeq number(s): NP_116573.1, NP_001614.3

Specificity: This antibody is expected to recognise isoform 1 (NP_116573.1) and isoform 3 (NP_001614.3). Human, Dog

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: Approx 16kDa band observed in Human Lymph Node and Spleen lysates (calculated MW of 16.7kDa according to NP_001614.3. Recommended concentration: 1-3µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN190906 (1µg/ml) staining of Human Lymph Node lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Kelemen, Autieri: "Expression of allograft inflammatory factor-1 in T lymphocytes: a role in T-lymphocyte activation and proliferative arteriopathies." in: The American journal of pathology, Vol. 167, Issue 2, pp. 619-26, 2005 (PubMed).