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Antibody
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anti-Integrin alpha M ITGAM
Integrin alpha M (ITGAM) antibody
| Antigen |
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| Synonyms |
CR3A, MO1A, CD11B, MAC-1, MAC1A, SLEB6, MGC117044, CR3, MAC1, Cd11b, Ly-40, Mac-1, Mac-1a, CD11b/CD18, F730045J24Rik |
| Clonality |
Monoclonal (MEM-170) |
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Host
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Reactivity
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Human (109), Mouse (Murine) (93), Rat (Rattus) (20), Rabbit (17), Monkey (10), Cow (Bovine) (8), Dog (Canine) (6), Goat (4), Primates (3), Sheep (Ovine) (3), Cat (Feline) (1), Horse (Equine) (1), Mustelid (1), Pig (Porcine) (1)
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Application
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 Alternatives Flow Cytometry (FACS), Functional Studies (Func)
Flow Cytometry (FACS) (168), Immunohistochemistry (Frozen Sections) (IHC (fro)) (50), Immunoprecipitation (IP) (48), Western Blotting (WB) (15), Functional Studies (Func) (14), Immunohistochemistry (IHC) (14), Blocking Antibody (Inhibition) (13), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (9), Enzyme Immunoassay (EIA) (4), ELISA (3), Immunocytochemistry (ICC) (3), Immunofluorescence (IF) (2), Radioimmunoassay (RIA) (2)
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| Catalog no. |
ABIN192410 |
| Quantity |
0.1 mg (1 mg/ml) |
| Price |
260.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 3 to 5 Business days |
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Alternative name
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CD11b
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Immunogen
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Human granulocytes
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Format
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Azide Free
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Isotype
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IgG1 (Matching secondary antibodies)
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Clone
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MEM-170
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Description
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CD11b (integrin alphaM subunit) is a 165-170 kDa type I transmembrane glycoprotein thatnon-covalently associates with integrin beta2 subunit (CD18), expression of the CD11bchain on the cell surface requires the presence of the CD18 antigen. CD11b/CD18 integrin(Mac-1, CR3) is highly expressed on NK cells, neutrophils, monocytes and less onmacrophages. CD11b/CD18 integrin is implicated in various adhesive interactions ofmonocytes, macrophages and granulocytes, facilitating their diapedesis, as well as itmediates the uptake of complement coated particles, serving as a receptor for the iC3bfragment of the third complement component.
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Specificity
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The antibody MEM-170 recognizes CD11b antigen (Mac-1alpha), a 165-170 kDatransmembrane protein type 1 mainly expressed on monocytes, granulocytes and NK-cells.The antibody blocks (not completely) Mac-1-mediated neutrophil binding to fibrinogen.HLDA VI., WS Code A054
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Application Notes
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Functional Application: The antibody MEM-170 blocks (not completely) Mac-1-mediated neutrophil binding tofibrinogen.
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Concentration
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1 mg/ml
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Purity
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> 95% (by SDS-PAGE)
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Purification
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Purified from ascites by protein-G affinity chromatography.
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Buffer
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Azide free phosphate buffered saline (PBS), approx. pH 7.4, 0.2 µ m filter sterilized.
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Storage
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Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.
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Research Area
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Stem Cells, Hematopoietic Progenitors, Hematopoietic Stem Cells, Glia marker, CD Antigens, Surface Receptors of Immune Cells
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Restrictions
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For Research Use only
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Alternatives for antigen "Integrin alpha M (ITGAM)", type "Antibodies"
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Hosts
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Mouse (102), Rat (89), Rabbit (8), Goat (6)
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Reactivities
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Human (109), Mouse (Murine) (93), Rat (Rattus) (20), Rabbit (17), Monkey (10), Cow (Bovine) (8), Dog (Canine) (6), Goat (4), Primates (3), Sheep (Ovine) (3), Cat (Feline) (1), Horse (Equine) (1), Mustelid (1), Pig (Porcine) (1)
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Applications
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Flow Cytometry (FACS) (168), Immunohistochemistry (Frozen Sections) (IHC (fro)) (50), Immunoprecipitation (IP) (48), Western Blotting (WB) (15), Functional Studies (Func) (14), Immunohistochemistry (IHC) (14), Blocking Antibody (Inhibition) (13), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (9), Enzyme Immunoassay (EIA) (4), ELISA (3), Immunocytochemistry (ICC) (3), Immunofluorescence (IF) (2), Radioimmunoassay (RIA) (2)
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Conjugates
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FITC (38), PE (25), Biotin (22), APC (7), RPE (4), SPRD (4), APC/Cy7 (2), Cy5 (2), PE/Cy7 (2), APC,Cy7 (1), Alexa Fluor 647 (1), Alexa Fluor 700 (1), PE,Cy5 (1), PE,DY647 (1), PE-Dyomics 647 (1), RPE,Cy5 (1)
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