TNNT1 antibody (AA 205-218)

Catalog No. ABIN1943131

Product Details anti-TNNT1 Antibody

Target: TNNT1 (Slow Skeletal Troponin T (TNNT1))

Binding Specificity: AA 205-218

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This TNNT1 antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human TNNT1 / TNT. This antibody is expected to recognize all reported isoforms (NP_003274.3, NP_001119604.1, NP_001119605.1).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-EKAQELSDWIHQLE from an internal region of human TNNT1 / TNT (NP_003274.3, NP_001119604.1, NP_001119605.1). Percent identity by BLAST analysis: Hamster, Platypus (93%), Galago (86%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), Peptide-ELISA (1:128000)

Usage: Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Preliminary experiments gave an approx 50kD band in Human Skeletal Muscle lysates after 0.1 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 32.9kD according to NP_003274.3. The 50kD band was successfully blocked by incubation with the immunizing peptide.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for TNNT1

Target: TNNT1 (Slow Skeletal Troponin T (TNNT1))

Alternative Name: TNNT1 / TNT (TNNT1 Products)

Synonyms: ANM antibody, NEM5 antibody, STNT antibody, TNT antibody, TNTS antibody, TNNI1 antibody, AW146156 antibody, Tnt antibody, sTnT antibody, ssTnT antibody, Fang2 antibody, tnTs antibody, Tnnt antibody, zgc:193831 antibody, zgc:193865 antibody, troponin T1, slow skeletal type antibody, troponin I1, slow skeletal type antibody, troponin T1, skeletal, slow antibody, troponin T1, slow skeletal type S homeolog antibody, troponin T type 1 (skeletal, slow) antibody, TNNT1 antibody, TNNI1 antibody, Tnnt1 antibody, tnnt1.S antibody, tnnt1 antibody

Background: Name/Gene ID: TNNT1

Synonyms: TNNT1, STNT, TNT, TNTS, Troponin-T1, skeletal, slow, ANM, Troponin T1, skeletal, slow, NEM5

Gene ID: 7138

NCBI Accession: NP_003274, NP_001119604, NP_001119605