JunB antibody (pSer79)

Catalog No. ABIN196656

The Rabbit Polyclonal anti-JunB antibody (ABIN196656) specifically detects JunB in IF and IHC (p). The antibody is reactive with Human and Mouse samples.

Quick Overview for JunB antibody (pSer79) (ABIN196656)

Target: JunB (JUNB) (Jun B Proto-Oncogene (JUNB))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This JunB antibody is un-conjugated

Application: Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-JunB Antibody

Binding Specificity: pSer79

Specificity: JunB (phospho-Ser79) antibody detects endogenous levels of JunB only when phosphorylated at serine 79.

Purification: Immunoaffinity chromatography

Immunogen: Synthetic phosphopeptide derived from human JunB around the phosphorylation site of serine 79 (G-A-SP-L-K).

Application Details

Application Notes: Suitable for use in Immunohistochemistry (1: 50approx. 1: 100).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl containing 0.02 % Sodium Azide as preservative and 50 % glycerol as stabilizer

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody (in aliquots) at -20 °C.

Target Details for JunB

Target: JunB (JUNB) (Jun B Proto-Oncogene (JUNB))

Alternative Name: Jun-B

Background: The cJun proto-oncogene was first identified as the cellular homolog of the avian sarcoma virus vjun oncogene. JunB and JunD have been shown to be almost identical to cJun in their C terminal regions, which are involved in dimerization and DNA binding, whereas their N terminal domains, which are involved in transcriptional activation, diverge. JunB is a transcription factor involved in regulating gene activity following the primary growth factor response. It binds to the DNA sequence 5'-TGA[CG]TCA-3'.Synonyms: JUNB, Transcription factor jun-B

Gene ID: 3726

NCBI Accession: NP_002220

UniProt: P17275