JunB antibody (pSer259)

Catalog No. ABIN196659

The Rabbit Polyclonal anti-JunB antibody is suitable to detect JunB in samples from Human. It has been validated for WB and IHC (p).

Quick Overview for JunB antibody (pSer259) (ABIN196659)

Target: JunB (JUNB) (Jun B Proto-Oncogene (JUNB))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This JunB antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-JunB Antibody

Binding Specificity: pSer259

Specificity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. JunB (phospho-Ser259) antibody detects endogenous levels of JunB only when phosphorylated at serine 259.

Purification: Immunoaffinity chromatography.

Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human JunB around the phosphorylation site of serine 259 (P-V-SP-P-I).

Application Details

Application Notes: Suitable for use in Western blot (1: 500approx. 1: 1000) and Immunohistochemistry (1: 50approx. 1: 100).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody (in aliquots) at -20 °C.

Target Details for JunB

Target: JunB (JUNB) (Jun B Proto-Oncogene (JUNB))

Alternative Name: Jun-B

Background: The cJun proto-oncogene was first identified as the cellular homolog of the avian sarcoma virus vjun oncogene. JunB and JunD have been shown to be almost identical to cJun in their C terminal regions, which are involved in dimerization and DNA binding, whereas their N terminal domains, which are involved in transcriptional activation, diverge. JunB is a transcription factor involved in regulating gene activity following the primary growth factor response. It binds to the DNA sequence 5'-TGA[CG]TCA-3'.Synonyms: JUNB, Transcription factor jun-B

Gene ID: 3726

NCBI Accession: NP_002220

UniProt: P17275