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ZAP70 antibody (zeta-Chain (TCR) Associated Protein Kinase 70kDa) (pTyr493)

Details for Product anti-ZAP70 Antibody No. ABIN196887, Supplier: Login to see
Antigen
Alternatives
anti-Human ZAP70 antibody for Western Blotting
Epitope
pTyr493
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51
47
29
22
22
22
21
15
15
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13
4
4
4
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3
3
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3
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2
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2
2
2
1
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1
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1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
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1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
485
171
119
4
4
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2
Host
Rabbit
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150
15
8
2
Clonality
Polyclonal
Conjugate
This ZAP70 antibody is un-conjugated
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22
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14
10
7
5
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4
4
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1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
298
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157
90
72
47
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44
15
7
5
4
4
3
2
Supplier
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Immunogen The antiserum was produced against synthesized phosphopeptide derived from Human Zap-70 around the phosphorylation site of tyrosine 493 (S-Y-Yp-T-A).
Specificity This antibody detects endogenous levels of Zap-70 only when phosphorylated at Tyrosine 493.
Purification Immunoaffinity chromatography using epitope-specific phosphopeptide.
Alternative Name ZAP70 (ZAP70 Antibody Abstract)
Background ZAP70, a 70 kDa member of the Syk tyrosine kinase family, plays a central role in lymphocyte activation and development, and is implicated in several immune disorders. Mutations in ZAP70 gene results in a form of Severe Combined Immunodeficiency Syndrome (SCID) in humans. ZAP70 expression also defines a subset of Chronic Lymphocytic Leukemia (CLL) in patients with unmutated Ig gene and poor clinical course.Synonyms: 70 kDa zeta-associated protein, SRK, Syk-related tyrosine kinase, ZAP 70, ZAP-70
Gene ID 7535
NCBI Accession NP_001070
UniProt P43403
Research Area Signaling, Tags/Labels, Immunology, Cancer, CD Antigens, Surface Receptors of Immune Cells
Pathways TCR Signaling
Application Notes Western Blot 1/500-1/1000). Immunohistochemistry on Paraffin-Embedded Sections (1/50-1/100).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store the antibody (in aliquots) at -20 °C.
Supplier Images
 image for anti-ZAP70 antibody (zeta-Chain (TCR) Associated Protein Kinase 70kDa) (pTyr493) (ABIN196887) anti-zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) (pTyr493) antibody
 image for anti-ZAP70 antibody (zeta-Chain (TCR) Associated Protein Kinase 70kDa) (pTyr493) (ABIN196887) anti-zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) (pTyr493) antibody (Image 2)
Background publications Salomon, Ficarro, Brill et al.: "Profiling of tyrosine phosphorylation pathways in human cells using mass spectrometry." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 2, pp. 443-8, 2003 (PubMed).

Kong, Dalton, Bubeck Wardenburg et al.: "Distinct tyrosine phosphorylation sites in ZAP-70 mediate activation and negative regulation of antigen receptor function." in: Molecular and cellular biology, Vol. 16, Issue 9, pp. 5026-35, 1996 (PubMed).

Chan, Dalton, Johnson et al.: "Activation of ZAP-70 kinase activity by phosphorylation of tyrosine 493 is required for lymphocyte antigen receptor function." in: The EMBO journal, Vol. 14, Issue 11, pp. 2499-508, 1995 (PubMed).