ZAP70 antibody (pTyr493)

Catalog No. ABIN196887

The Rabbit Polyclonal anti-ZAP70 antibody is suitable to detect ZAP70 in samples from Human, Mouse and Rat. It has been validated for WB and IHC (p).

Quick Overview for ZAP70 antibody (pTyr493) (ABIN196887)

Target: ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ZAP70 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-ZAP70 Antibody

Binding Specificity: pTyr493

Specificity: This antibody detects endogenous levels of Zap-70 only when phosphorylated at Tyrosine 493.

Purification: Immunoaffinity chromatography using epitope-specific phosphopeptide.

Immunogen: The antiserum was produced against synthesized phosphopeptide derived from Human Zap-70 around the phosphorylation site of tyrosine 493 (S-Y-Yp-T-A).

Application Details

Application Notes: Western Blot 1/500-1/1000). Immunohistochemistry on Paraffin-Embedded Sections (1/50-1/100).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody (in aliquots) at -20 °C.

Target Details for ZAP70

Target: ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))

Alternative Name: ZAP70

Background: ZAP70, a 70 kDa member of the Syk tyrosine kinase family, plays a central role in lymphocyte activation and development, and is implicated in several immune disorders. Mutations in ZAP70 gene results in a form of Severe Combined Immunodeficiency Syndrome (SCID) in humans. ZAP70 expression also defines a subset of Chronic Lymphocytic Leukemia (CLL) in patients with unmutated Ig gene and poor clinical course.Synonyms: 70 kDa zeta-associated protein, SRK, Syk-related tyrosine kinase, ZAP 70, ZAP-70

Gene ID: 7535

NCBI Accession: NP_001070

UniProt: P43403

Pathways: TCR Signaling, Ubiquitin Proteasome Pathway