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Antibody
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anti-Activating Transcription Factor 2 ATF2
Activating Transcription Factor 2 (ATF2) antibody
| Antigen |
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| Synonyms |
HB16, CREB2, TREB7, CRE-BP1, MGC111558, ATF2, MGC142504, CHN1, CG12850, CG3749, dATF-2, DmelCG30420, CG30420 |
| Clonality |
Polyclonal |
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Host
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Reactivity
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Application
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 Alternatives Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB), Immunofluorescence (IF)
Western Blotting (WB) (104), Immunohistochemistry (IHC) (63), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (46), Immunofluorescence (IF) (36), Immunoprecipitation (IP) (32), Enzyme Immunoassay (EIA) (30), ELISA (24), Flow Cytometry (FACS) (14), Dot Blot (Dot) (8), Immunohistochemistry (Frozen Sections) (IHC (fro)) (7), ELISA (Detection) (2), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoelectron Microscopy (EM) (1)
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| Catalog no. |
ABIN197149 |
| Quantity |
50 µg (1.0 mg/ml) (Variants) |
| Price |
200.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 7 to 10 Business Days |
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Characteristics
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Synonyms: ATF-2, CREB2, CREBP1, Activating transcription factor 2, CRE-BP1, HB16, CREB-2, CyclicAMP-dependent transcription factor ATF-2, cAMP-dependent transcription factor ATF-2,cAMP response element-binding protein CRE-BP1, Cyclic AMP-responsive element-bindingprotein 2
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Alternative name
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ATF2
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Swiss-Prot
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P15336
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Immunogen
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The antiserum was produced against synthesized non-phosphopeptide derived fromhuman ATF-2 around the phosphorylation site of threonine 71 or 53 (T-P-TP-P-T).
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Description
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ATF2 (Activating Transcription Factor 2, CREBP, HB16, CREB2, TREB7) is a member of theATF/CREB family of basic region leucine zipper DNA binding proteins that regulatestranscription by binding to a consensus cAMP response element (CRE) in the promoter ofvarious viral and cellular genes. Many of these genes are important in cell growth anddifferentiation, and in stress and immune responses. ATF2 is a nuclear protein that bindsDNA as a dimer and can form dimers with members of the ATF/CREB and Jun/Fos families. It is a stronger activator as a heterodimer with cJun than as a homodimer. Several isoformsof ATF2 arise by differential splicing. The stable native full length ATF2 is transcriptionallyinactive as a result of an inhibitory direct intramolecular interaction of its carboxy terminalDNA binding domain with the amino terminal transactivation domain. Followingdimerization ATF2 becomes a short lived protein that undergoes ubiquitination andproteolysis, seemingly in a protein phosphatase-dependent mechanism. Stimulation of thetranscriptional activity of ATF2 occurs following cellular stress induced by severalgenotoxic agents, inflammatory cytokines, and UV irradiation. This activation requiresphosphorylation of two threonine residues in ATF2 by both JNK/SAP kinase and p38 MAPkinase. ATF2 is abundantly expressed in brain.
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Specificity
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ATF-2 antibody detects endogenous levels of total ATF-2 protein. Species: Human, Mouse, Rat. Others not tested.
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Application Notes
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Western Blot: 1: 500~1: 1000. Immunohistochemistry: 1: 50~1: 100. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
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Concentration
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1.0 mg/ml
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Buffer
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PBS(without Mg2+ and Ca2+), pH 7.4 containing 150mM NaCl, 0.02%sodium azide and 50% glycerol
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Storage
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Store the antibody at -20°C. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
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Research Area
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Immunology, Innate Immunity, Transcription Factors
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Restrictions
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For Research Use only
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Alternatives for antigen "Activating Transcription Factor 2 (ATF2)", type "Antibodies"
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Hosts
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Rabbit (142), Mouse (16), Goat (4), Sheep (3)
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Reactivities
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Human (162), Mouse (Murine) (111), Rat (Rattus) (99), Cow (Bovine) (1), Dog (Canine) (1), Horse (Equine) (1)
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Applications
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Western Blotting (WB) (104), Immunohistochemistry (IHC) (63), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (46), Immunofluorescence (IF) (36), Immunoprecipitation (IP) (32), Enzyme Immunoassay (EIA) (30), ELISA (24), Flow Cytometry (FACS) (14), Dot Blot (Dot) (8), Immunohistochemistry (Frozen Sections) (IHC (fro)) (7), ELISA (Detection) (2), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoelectron Microscopy (EM) (1)
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Conjugates
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Alexa Flour 488 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy7 (1), RBITC (1)
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Epitopes
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pThr69/51 (6), Ab-112 or 94 (5), Ab-62 or 44 (5), Ab-69 or 51 (5), Ab-71 or 53 (5), Ab-73 or 55 (5), pSer112 or 94 (5), pSer62 or 44 (5), pSer62/44 (5), pThr69 or 51 (5), pThr71 or 53 (5), pThr73 or 55 (5), pSer112/94 (4), pThr71/53 (4), pThr73/55 (3), S44 (2), S94 (2), PSer340/322 (1), S322 (1), pSer44 (1), pSer472 (1), pSer94 (1), pThr51 (1), pThr69/51 + pThr71/53 (1), pThr71 (1), pThr79/51 (1)
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