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ZAP70 antibody (Tyr319)

The Rabbit Polyclonal anti-ZAP70 antibody is suitable to detect ZAP70 in samples from Human and Mouse. It has been validated for WB and IHC (p).

Image no. 1 for anti-zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) (Tyr319) antibody (ABIN197384) (ZAP70 antibody (Tyr319))

2 images

Catalog No. ABIN197384

$588.00

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Quick Overview for ZAP70 antibody (Tyr319) (ABIN197384)

Target

See all ZAP70 Antibodies

ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))

Reactivity

Human, Mouse

Host

Rabbit

Clonality

Polyclonal

Conjugate

This ZAP70 antibody is un-conjugated

Application

Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Discover our top product ZAP70 Primary Antibody

Binding Specificity
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28

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20

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Tyr319

Specificity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. Zap-70 antibody AP02691PU detects endogenous levels of total Zap-70 protein.

Purification

Immunoaffinity chromatography.

Immunogen

The antiserum was produced against synthesized non-phosphopeptide derived from human Zap-70 around the phosphorylation site of tyrosine 319 (S-P-Yp-S-D).
anti-zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) (C-Term) antibody
ZAP70 Reactivity: Human WB, ICC, ICFC Host: Mouse Monoclonal ZAP-03 unconjugated

anti-zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) (pTyr319) antibody
ZAP70 Reactivity: Human, Mouse, Rat WB, IHC, ELISA, IF, ICC Host: Rabbit Polyclonal unconjugated

anti-zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) (AA 247-382) antibody
ZAP70 Reactivity: Human IHC, ELISA, FACS, IF Host: Mouse Monoclonal ZAP70-2035 unconjugated

anti-zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) (AA 169-390) antibody
ZAP70 Reactivity: Human WB, ELISA, FACS Host: Mouse Monoclonal 1F3G9 unconjugated

anti-zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) (AA 337-600) antibody
ZAP70 Reactivity: Human WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

anti-zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) (pTyr493) antibody
ZAP70 Reactivity: Human, Mouse, Rat WB, IHC, ELISA, IF, ICC Host: Rabbit Polyclonal unconjugated

anti-zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) (AA 1-254) antibody
ZAP70 Reactivity: Human IHC, FACS, IF Host: Mouse Monoclonal 2F3-2 unconjugated

anti-zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) (AA 169-390) antibody
ZAP70 Reactivity: Human WB, ELISA Host: Mouse Monoclonal 6D9C2 unconjugated

anti-zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) (AA 247-382) antibody
ZAP70 Reactivity: Human IHC, ELISA Host: Mouse Monoclonal ZAP70-2046 unconjugated

anti-zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) antibody
ZAP70 Reactivity: Human WB, IF Host: Rabbit Monoclonal unconjugated

Application Notes

Suitable for use in Western blot (1: 500-1: 1000) and Immunohistochemistry (1: 50-1: 100).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions

For Research Use only
Concentration

1.0 mg/mL

Buffer

PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.

Preservative

Sodium azide

Precaution of Use

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice

Avoid repeated freezing and thawing.

Storage

-20 °C

Storage Comment

Store the antibody (in aliquots) at -20 °C.
Target

ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))

Alternative Name

ZAP70

Background

Synonyms: 70 kDa zeta-associated protein, SRK, Syk-related tyrosine kinase, ZAP 70, ZAP-70

Gene ID

7535

NCBI Accession

NP_001070

UniProt

P43403

Pathways

TCR Signaling, Ubiquitin Proteasome Pathway