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Amyloid beta (A4) Precursor Protein (APP) (AA 666-670) antibody

Details for Product No. ABIN197432, Supplier: Login to see
Antigen
  • APP
  • APP-like
  • APPL
  • Abeta
  • BcDNA:GH04413
  • CG7727
  • Dmel\\CG7727
  • EG:65F1.5
  • appl
  • aaa
  • abeta
  • abpp
  • ad1
  • appi
  • ctfgamma
  • cvap
  • pn2
  • AAA
  • ABETA
  • ABPP
  • AD1
  • APPI
  • CTFgamma
  • CVAP
  • PN-II
  • PN2
  • Abpp
  • Adap
  • Ag
  • Cvap
  • E030013M08Rik
  • betaApp
  • app
  • wu:fj34d10
  • wu:fk65e12
  • zgc:85740
Alternatives
anti-Human Amyloid beta (A4) Precursor Protein antibody for Enzyme Immunoassay
Epitope
AA 666-670
122
109
52
35
25
20
19
17
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8
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3
3
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3
3
3
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2
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2
2
2
2
2
2
2
2
2
2
2
1
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1
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1
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Reactivity
Human, Mouse (Murine), Rat (Rattus)
823
307
276
41
11
9
8
8
6
3
2
2
1
1
1
1
Host
Rabbit
579
223
50
3
2
Clonality
Polyclonal
Conjugate
Un-conjugated
48
37
35
17
13
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11
2
2
1
Application
Immunofluorescence (IF), Western Blotting (WB)
563
316
231
150
147
74
57
33
29
20
15
10
9
8
6
3
2
2
2
2
1
Supplier
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Available images

'Independent Validation' Badge
Lot Number 8715
Method validated Immunofluorescence
Positive Control Human brain
Negative Control Human liver
Notes Signal was detected in positive control tissue, and no signal was seen in negative control tissue.
Primary Antibody
  • Antibody: Amyloid beta A4 precursor protein (APP)
  • Catalog number: ABIN197433
  • Supplier: Login to see
  • Supplier catalog number: Login to see
  • Lot number: 8715
Isotype Control Antibody
Secondary Antibody
Controls
  • Positive control: human brain (specimen known to contain the target protein) from Molecular Pathology
  • Core.
  • Negative Control: human liver (specimen known to not contain the target protein or express low level)
  • from Molecular Pathology Core.
  • Primary antibody isotype control: human brain treated with primary antibody isotype control instead of
  • the primary antibody.
  • Secondary antibody only control: human brain treated with secondary antibody only (no primary
  • antibody).
Protocol
  • Sections were deparaffinized and rehydrated.
  • Sections were heated to 98°C for 20 min in citrate buffer pH 6.0 (Biogenex, HK086-9K) for antigen retrieval and cooled down for 20 min on the bench.
  • Sections were blocked in 10% NGS (normal goat serum) for 20 min at room temperature.
  • Sections were washed x 2 in 1xTBS buffer.
  • Sections were incubated with primary antibody diluted 1:100 in Antibody Diluent (Invitrogen, 003218) at 4°C overnight.
  • Sections were washed x 2 in 1xTBS buffer.
  • Sections were incubated with AlexaFluor 488 Goat anti-Rabbit IgG 1:1000 for 60 min.
  • Sections were washed x 3 in 1xTBS buffer.
  • Sections were mounted with DAPI (Invitrogen, Prolong Gold antifade reagent with DAPI) and coverslipped.
  • Sections were photographed with a Zeiss Axioskop2 microscope
Experimental Notes None
Validation Images
Immunofluorescence validation image for anti-Amyloid beta (A4) Precursor Protein (APP) (AA 666-670) antibody (ABIN197433) Figure 1. Micrograph image of positive control (human brain FFPE tissue). APP stainin...
Immunofluorescence validation image for anti-Amyloid beta (A4) Precursor Protein (APP) (AA 666-670) antibody (ABIN197433) Figure 2: micrograph image of negative control (human liver FFPE tissue) stained with...
Immunofluorescence validation image for anti-Amyloid beta (A4) Precursor Protein (APP) (AA 666-670) antibody (ABIN197433) Figure 3: micrograph image of isotype control (rabbit IgG isotype control antibody on...
Immunofluorescence validation image for anti-Amyloid beta (A4) Precursor Protein (APP) (AA 666-670) antibody (ABIN197433) Figure 4: micrograph image of secondary antibody only control (no primary antibody on...
Immunogen peptide Sequence around amino acids 666~670 (A-V-T-P-E) derived from Human APP protein.
Specificity This antibody detects endogenous levels of total APP protein.
Purification Affinity Chromatography using epitope-specific immunogen.
Alternative Name Amyloid beta A4 Protein / APP (APP Antibody Abstract)
Background Amyloid beta precursor protein gene (ABPP) encodes a cell surface receptor and transmembrane precursor protein that is cleaved by secretases to form a number of peptides. Multiple transcript variants encoding several different isoforms have been found for this gene. Isoform APP695 is the predominant form in neuronal tissue, isoform APP751 and isoform APP770 are widely expressed in nonneuronal cells. Isoform APP751 is the most abundant form in T lymphocytes. ABPP is expressed in all fetal tissues examined with the highest levels in brain, kidney, heart and spleen with weak expression observed in liver, ABPP is induced during neuronal differentiation. In the adult brain, highest expression of ABPP gene is found in the frontal lobe of the cortex and in the anterior perisylvian cortex opercular gyri, moderate expression in the cerebellar cortex, the posterior perisylvian cortex opercular gyri and the temporal associated cortex. Weak expression is found in the striate, extra striate and motor cortices. Mutations in ABPP have been implicated in autosomal dominant Alzheimer disease and cerebroarterial amyloidosis (cerebral amyloid angiopathy).Synonyms: ABPP, APPI, Alzheimer disease amyloid protein, Amyloid Precursor Protein, CVAP, Cerebral vascular amyloid peptide, PreA4, Protease nexin-II
Gene ID 351
NCBI Accession NP_000475
UniProt P05067
Research Area Neurology, Alzheimer's Disease
Pathways Caspase Cascade in Apoptosis, EGFR Signaling Pathway
Application Notes Western Blot: 1/500-1/1000. Immunofluorescence: 1/100-1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer PBS (without Mg2+ and Ca2+), pH 7.4 containing 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store the antibody (in aliquots) at -20 °C.
Supplier Images
 image for anti-Amyloid beta (A4) Precursor Protein (APP) (AA 666-670) antibody (ABIN197432) anti-Amyloid beta (A4) Precursor Protein (APP) (AA 666-670) antibody
 image for anti-Amyloid beta (A4) Precursor Protein (APP) (AA 666-670) antibody (ABIN197432) anti-Amyloid beta (A4) Precursor Protein (APP) (AA 666-670) antibody (Image 2)
Background publications Iijima, Ando, Takeda et al.: "Neuron-specific phosphorylation of Alzheimer's beta-amyloid precursor protein by cyclin-dependent kinase 5." in: Journal of neurochemistry, Vol. 75, Issue 3, pp. 1085-91, 2000 (PubMed).

Eng, Lund, Campenot: "Synthesis of beta-tubulin, actin, and other proteins in axons of sympathetic neurons in compartmented cultures." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 19, Issue 1, pp. 1-9, 1999 (PubMed).

Hung, Selkoe: "Selective ectodomain phosphorylation and regulated cleavage of beta-amyloid precursor protein." in: The EMBO journal, Vol. 13, Issue 3, pp. 534-42, 1994 (PubMed).

Suzuki, Oishi, Marshak et al.: "Cell cycle-dependent regulation of the phosphorylation and metabolism of the Alzheimer amyloid precursor protein." in: The EMBO journal, Vol. 13, Issue 5, pp. 1114-22, 1994 (PubMed).