TNF alpha antibody (FITC)

Catalog No. ABIN1981885

Product Details anti-TNF alpha Antibody (FITC)

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Reactivity: Human, Pig, Non-Human Primate

Host: Mouse

Clonality: Monoclonal

Conjugate: This TNF alpha antibody is conjugated to FITC

Application: Intracellular Flow Cytometry (ICFC)

Purpose: Anti-TNF alpha FITC

Specificity: The mouse monoclonal antibody MAb11 recognizes human 17-26 kDa cytokine TNF-alpha (tumor necrosis factor alpha).

Cross-Reactivity (Details): Human, Non-Human Primates, Porcine

Purification: Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogen: Recombinant human TNF-alpha

Clone: MAb11

Isotype: IgG1 kappa

Application Details

Application Notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests. Intracellular staining.

Restrictions: For Research Use only

Handling

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze. Avoid prolonged exposure to light.
Do not use after expiration date stamped on vial label.
Short-term exposure to room temperature should not affect the quality of the reagent. However, if reagent is stored under any conditions other than those specified, the conditions must be verified by the user.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

References for anti-TNF alpha antibody (FITC) (ABIN1981885)

Cesaro-Tadic, Dernick, Juncker, Buurman, Kropshofer, Michel, Fattinger, Delamarche: "High-sensitivity miniaturized immunoassays for tumor necrosis factor alpha using microfluidic systems." in: Lab on a chip, Vol. 4, Issue 6, pp. 563-9, (2004) (PubMed).

Yan, Qing, Byers, Stadnyk, Al-Hertani, Bortolussi: "Role of MyD88 in diminished tumor necrosis factor alpha production by newborn mononuclear cells in response to lipopolysaccharide." in: Infection and immunity, Vol. 72, Issue 3, pp. 1223-9, (2004) (PubMed).

Attarbaschi, Willheim, Ramharter, Hofmann, Wahl, Winkler, Graninger, Winkler: "T cell cytokine profile during primary Epstein-Barr virus infection (infectious mononucleosis)." in: European cytokine network, Vol. 14, Issue 1, pp. 34-9, (2003) (PubMed).

Visser, Graffelman, Blauw, Haspels, Lentjes, de Kloet, Nagelkerken: "LPS-induced IL-10 production in whole blood cultures from chronic fatigue syndrome patients is increased but supersensitive to inhibition by dexamethasone." in: Journal of neuroimmunology, Vol. 119, Issue 2, pp. 343-9, (2001) (PubMed).

Wahlström, Katchar, Wigzell, Olerup, Eklund, Grunewald: "Analysis of intracellular cytokines in CD4+ and CD8+ lung and blood T cells in sarcoidosis." in: American journal of respiratory and critical care medicine, Vol. 163, Issue 1, pp. 115-21, (2001) (PubMed).

Target Details for TNF alpha

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Alternative Name: TNF alpha (TNF alpha Products)

Background: Tumor necrosis factor,TNF-alpha is a cytokine produced by monocytes, macrophages, neutrophils, NK cells, CD4+ T cells and many transformed cells. It can be expressed as a 17 kDa free molecule, or as a 26 kDa membrane protein. TNF-alpha easily forms stable trimers, but also other multimeric complexes. In the immune system, it is an important regulator, which has cytolytic and cytostatic activity against a range of tumor cells, increases fibroblast proliferation and supports neutrophil chemotaxis and phagocytosis.,TNFA, DIF, TNLG1F

Gene ID: 7124

UniProt: P01375

Pathways: NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome