GLN1 antibody (AA 300-350)

Catalog No. ABIN2017584

Product Details anti-GLN1 Antibody

Target: GLN1 (Glutamine Synthetase (GLN1))

Binding Specificity: AA 300-350

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GLN1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Specificity: Rabbit Anti-Glutamine Synthetase Polyclonal Antibody detects endogenous levels of human, mouse and rat Glutamine Synthetase protein.

Purification: Immunoaffinity chromatography

Immunogen: KLH-coupled synthetic peptide from within AA 300-350 of Human Glutamine Synthetase protein .

Isotype: IgG

Application Details

Application Notes: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

ELISA: 0.05-0.2 µg/mL
Western blot: 0.5-1 µg/mLImmunohistochemistry: 5-10 µg/mLOther Applications: user-optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: PBS, pH 7.4, containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Storage: 4 °C/-20 °C

Storage Comment: The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Target Details for GLN1

Target: GLN1 (Glutamine Synthetase (GLN1))

Alternative Name: Glutamine Synthetase (GLN1 Products)

Synonyms: GLNS antibody, GS antibody, PIG43 antibody, PIG59 antibody, Glns antibody, glutamate-ammonia ligase antibody, glutamate-ammonia ligase (glutamine synthetase) antibody, glutamine synthetase antibody, GLUL antibody, Glul antibody, GLN1 antibody

Background: Glutamine synthetase is an enzyme that plays an essential role in the metabolism of nitrogen by catalyzing the condensation of glutamate and ammonia to form glutamine. Glutamine synthetase activity is a useful marker for astrocytes and an important differentiation feature in retina. It is also considered to be a key enzyme in the recycling of the neurotransmitter glutamate.Rabbit Anti-Glutamine Synthetase Polyclonal Antibody is developed in rabbit using a KLH-coupled synthetic peptide from within residues 300-350 of human Glutamine Synthetase protein (Swiss Prot: P15104).

Pathways: Positive Regulation of Peptide Hormone Secretion