APP antibody (C-Term)

Catalog No. ABIN205860

The Rabbit Polyclonal anti-APP antibody (ABIN205860) specifically detects APP in WB, IHC and IHC (p). The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for APP antibody (C-Term) (ABIN205860)

Target: APP (Amyloid beta (A4) Precursor Protein (APP))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This APP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-APP Antibody

Binding Specificity: AA 737-751, C-Term

Specificity: Recognizes both intact Amyloid Precursor Protein (APP), and also the C99 fragment generated by Beta-secretase. The sequence recognized by this corresponds to amino acids 85-99 of the C99 fragment. The C99 fragment itself is a substrate for gamma-secretase to generate the 4 kD beta amyloid peptide, found in the brains of Alzheimer's disease patients. APP also inhibits Notch signaling through it's interaction with NUMB. Has been used successfully for the detection of the ~14 kD beta C-terminal fragment of APP, produced as a result of cleavage by BACE1 using western blotting -in rat retinal lysates.

Purification: Purified

Immunogen: Synthetic peptide corresponding to amino acids 737-751 of human APP ( C-TERMINAL ).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P, WB (1:500 - 1:1000)

Usage: Immunohistology: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Target Details for APP

Target: APP (Amyloid beta (A4) Precursor Protein (APP))

Alternative Name: APP / beta Amyloid

Background: Name/Gene ID: APP
Family: Amyloid precursor protein

Synonyms: APP, ABPP, A4, ABETA, AD1, Alzheimer disease, Amyloid beta, Beta-amyloid peptide, APPI, Beta amyloid, CVAP, AAA, PN2, PreA4, Amyloid beta A4 protein, Peptidase nexin-II, CTFgamma, PN-II, Protease nexin-II

Gene ID: 351

UniProt: P05067

Pathways: Caspase Cascade in Apoptosis, EGFR Signaling Pathway, Transition Metal Ion Homeostasis, Skeletal Muscle Fiber Development, Toll-Like Receptors Cascades, Feeding Behaviour