Band 3/AE1 antibody (AA 184-233)

Catalog No. ABIN6736430

Product Details anti-Band 3/AE1 Antibody

Target: Band 3/AE1 (SLC4A1) (Solute Carrier Family 4, Anion Exchanger, Member 1 (erythrocyte Membrane Protein Band 3, Diego Blood Group) (SLC4A1))

Binding Specificity: AA 184-233

Reactivity: Human, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Band 3/AE1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human SLC4A1 / Band 3

Predicted Reactivity: Percent identity by BLAST analysis: Human (100%) Rabbit (84%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa184-233 of human CHAMP1 (P02730, NP_000333). Percent identity by BLAST analysis: Human, Gorilla, Gibbon (100%), Orangutan, Bat (92%), Marmoset (91%), Rabbit (84%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.Antibody has been tested in a peptide-based ELISA.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: After adding water, will consist of PBS buffer with 2 % sucrose

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for Band 3/AE1

Target: Band 3/AE1 (SLC4A1) (Solute Carrier Family 4, Anion Exchanger, Member 1 (erythrocyte Membrane Protein Band 3, Diego Blood Group) (SLC4A1))

Alternative Name: SLC4A1 / Band 3 / AE1 (SLC4A1 Products)

Synonyms: AE1 antibody, BND3 antibody, CD233 antibody, DI antibody, EMPB3 antibody, EPB3 antibody, FR antibody, RTA1A antibody, SW antibody, WD antibody, WD1 antibody, WR antibody, Ae1 antibody, Empb3 antibody, l11Jus51 antibody, ae1 antibody, band3 antibody, MGC152771 antibody, zgc:111889 antibody, zgc:152771 antibody, si:dz180g5.1 antibody, LOC100136769 antibody, slc4a1 antibody, wd1 antibody, bnd3 antibody, epb3 antibody, cd233 antibody, empb3 antibody, rta1a antibody, MGC80391 antibody, SLC4A1 antibody, BB3 antibody, EAT antibody, solute carrier family 4 member 1 (Diego blood group) antibody, solute carrier family 4 (anion exchanger), member 1 antibody, solute carrier family 4 (anion exchanger), member 1a (Diego blood group) antibody, Band 3 antibody, erythrocyte membrane protein band 4.1 like 3 antibody, solute carrier family 4 member 1 (Diego blood group) L homeolog antibody, solute carrier family 4 member 1 antibody, SLC4A1 antibody, Slc4a1 antibody, slc4a1a antibody, LOC100136769 antibody, EPB41L3 antibody, slc4a1.L antibody, slc4a1 antibody

Background: Name/Gene ID: SLC4A1
Subfamily: Anion exchanger
Family: Transporter

Synonyms: SLC4A1, Anion exchanger 1, Anion exchanger-1, AE1, Anion exchange protein 1, Band 3, Band 3 anion transport protein, BND3, CD233, DI, EPB3, AE 1, Froese blood group, FR, RTA1A, SW, Swann blood group, WD1, WR, Wright blood group, WD, CD233 antigen, EMPB3, Waldner blood group

Gene ID: 6521

NCBI Accession: NP_000333

UniProt: P02730